Transcriptome profiling of grapevine seedless segregants during berry development reveals candidate genes associated with berry weight

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dc.contributor.authorMuñoz-Espinoza, Claudia
dc.contributor.authorDi Genova, Alex
dc.contributor.authorCorrea, José
dc.contributor.authorSilva, Romina
dc.contributor.authorMaass, Alejandro
dc.contributor.authorGonzález-Agüero, Mauricio
dc.contributor.authorOrellana, Ariel
dc.contributor.authorHinrichsen, Patricio
dc.date.accessioned2016-07-28T20:39:19Z
dc.date.available2016-07-28T20:39:19Z
dc.date.issued2016-04
dc.descriptionIndexación: Web of Science; PubMedes
dc.description.abstractBackground Berry size is considered as one of the main selection criteria in table grape breeding programs. However, this is a quantitative and polygenic trait, and its genetic determination is still poorly understood. Considering its economic importance, it is relevant to determine its genetic architecture and elucidate the mechanisms involved in its expression. To approach this issue, an RNA-Seq experiment based on Illumina platform was performed (14 libraries), including seedless segregants with contrasting phenotypes for berry weight at fruit setting (FST) and 6–8 mm berries (B68) phenological stages. Results A group of 526 differentially expressed (DE) genes were identified, by comparing seedless segregants with contrasting phenotypes for berry weight: 101 genes from the FST stage and 463 from the B68 stage. Also, we integrated differential expression, principal components analysis (PCA), correlations and network co-expression analyses to characterize the transcriptome profiling observed in segregants with contrasting phenotypes for berry weight. After this, 68 DE genes were selected as candidate genes, and seven candidate genes were validated by real time-PCR, confirming their expression profiles. Conclusions We have carried out the first transcriptome analysis focused on table grape seedless segregants with contrasting phenotypes for berry weight. Our findings contributed to the understanding of the mechanisms involved in berry weight determination. Also, this comparative transcriptome profiling revealed candidate genes for berry weight which could be evaluated as selection tools in table grape breeding programs.es
dc.description.urihttp://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-016-0789-1
dc.identifier.citationBMC Plant BiologyBMC series – open, inclusive and trusted 2016 16:104es
dc.identifier.issn1471-2229
dc.identifier.otherDOI: 10.1186/s12870-016-0789-1
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/1565
dc.language.isoenes
dc.publisherBIOMED CENTRALes
dc.subjectRNA-seqes
dc.subjectTable grapeses
dc.subjectBerry weightes
dc.subjectFunctional genomicses
dc.subjectCandidate geneses
dc.subjectVITIS-VINIFERA L.es
dc.subjectQUANTITATIVE TRAIT LOCIes
dc.subjectRNA-SEQes
dc.subjectCABERNET-SAUVIGNONes
dc.subjectPROTEOMIC ANALYSISes
dc.subjectPROTEIN-KINASESes
dc.subjectR-PACKAGEes
dc.subjectIDENTIFICATIONes
dc.subjectEXPRESSIONes
dc.subjectARABIDOPSISes
dc.titleTranscriptome profiling of grapevine seedless segregants during berry development reveals candidate genes associated with berry weightes
dc.typeArtículoes
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