Restraint stress increases hemichannel activity in hippocampal glial cells and neurons

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dc.contributor.authorOrellana, Juan A.
dc.contributor.authorMoraga-Amaro, Rodrigo
dc.contributor.authorDíaz-Galarce, Raúl
dc.contributor.authorRojas, Sebastián
dc.contributor.authorMaturana, Carola J.
dc.contributor.authorStehberg, Jimmy
dc.contributor.authorSáez, Juan C.
dc.date.accessioned2023-05-02T21:22:22Z
dc.date.available2023-05-02T21:22:22Z
dc.date.issued2015-04
dc.descriptionIndexación: Scopuses
dc.description.abstractStress affects brain areas involved in learning and emotional responses, which may contribute in the development of cognitive deficits associated with major depression. These effects have been linked to glial cell activation, glutamate release and changes in neuronal plasticity and survival including atrophy of hippocampal apical dendrites, loss of synapses and neuronal death. Under neuro-inflammatory conditions, we recently unveiled a sequential activation of glial cells that release ATP and glutamate via hemichannels inducing neuronal death due to activation of neuronal NMDA/P2X7 receptors and pannexin1 hemichannels. In the present work, we studied if stressinduced glia activation is associated to changes in hemichannel activity. To this end, we compared hemichannel activity of brain cells after acute or chronic restraint stress in mice. Dye uptake experiments in hippocampal slices revealed that acute stress induces opening of both Cx43 and Panx1 hemichannels in astrocytes, which were further increased by chronic stress; whereas enhanced Panx1 hemichannel activity was detected in microglia and neurons after acute/chronic and chronic stress, respectively. Moreover, inhibition of NMDA/P2X7 receptors reduced the chronic stress-induced hemichannel opening, whereas blockade of Cx43 and Panx1 hemichannels fully reduced ATP and glutamate release in hippocampal slices from stressed mice. Thus, we propose that gliotransmitter release through hemichannels may participate in the pathogenesis of stress-associated psychiatric disorders and possibly depression. © 2015 Orellana, Moraga-Amaro, Díaz-Galarce, Rojas, Maturana, Stehberg and Sáez.es
dc.description.urihttps://www.frontiersin.org/articles/10.3389/fncel.2015.00102/full
dc.identifier.citationFrontiers in Cellular Neuroscience Open Access Volume 9, Issue APR, Pages 1 - 122 April 2015es
dc.identifier.doi10.3389/fncel.2015.00102
dc.identifier.issn1662-5102
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/49182
dc.language.isoenes
dc.publisherFrontiers Media S.A.es
dc.rights.licenseAtribución 4.0 Internacional (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectConnexinses
dc.subjectGliaes
dc.subjectHemichannelses
dc.subjectHippocampuses
dc.subjectNeurones
dc.subjectPannexinses
dc.subjectStresses
dc.titleRestraint stress increases hemichannel activity in hippocampal glial cells and neuronses
dc.typeArtículoes
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