Antisense noncoding mitochondrial RNA-2 gives rise to miR-4485-3p by Dicer processing in vitro

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dc.contributor.authorFarfán, Nicole
dc.contributor.authorSanhueza, Nicole
dc.contributor.authorBriones, Macarena
dc.contributor.authorBurzio, Luis O.
dc.contributor.authorBurzio, Verónica A.
dc.date.accessioned2022-06-06T14:50:55Z
dc.date.available2022-06-06T14:50:55Z
dc.date.issued2021-12
dc.descriptionIndexación Scopuses
dc.description.abstractBackground: The antisense noncoding mitochondrial RNAs (ASncmtRNAs) derive from the mitochondrial 16S gene. Knockdown of these transcripts with chemically-modified antisense oligonucleotides induces proliferative arrest, apoptosis and invasiveness reduction in tumor but not normal cells. One of these transcripts, ASncmtRNA-2, contains the complete and identical sequence of hsa-miR-4485-3p and, upon knockdown of this transcript, there is a strong increase in levels of this miRNA, suggesting ASncmtRNA-2 as a source for miR-4485-3p, which is supported by several evidences from our group and others, in the ex vivo setting. Results: Here we show that incubation of in vitro-transcribed ASncmtRNA-2 with recombinant Dicer produces RNA fragments corresponding to hsa-miR-4485-3p, showing that Dicer binds to and processes ASncmtRNA-2, strongly supporting the hypothesis that ASncmtRNA-2 acts as a precursor for miR-4485-3p. Conclusion: The in vitro results presented here strengthen the hypothesis that miR-4485-3p is derived from ASncmtRNA-2 by Dicer processing. Since miR-4485-3p is classified as a tumor suppressor miRNA, this evidence strengthens the application of ASncmtRNA knockdown for cancer therapy.en
dc.identifier.citationBiological Research Volume 54, Issue 1 December 2021 Article number 33en
dc.identifier.doi10.1186/s40659-021-00356-0
dc.identifier.issn07169760
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/22720
dc.language.isoenen
dc.publisherBioMed Central Ltden
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectCanceren
dc.subjectCloningen
dc.subjectDiceren
dc.subjectlncRNAen
dc.subjectmiRNAen
dc.subjectncRNAen
dc.titleAntisense noncoding mitochondrial RNA-2 gives rise to miR-4485-3p by Dicer processing in vitroen
dc.typeArtículoen
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