Role of SUMO-1 and SUMO interacting motifs in rhesus TRIM5α-mediated restriction

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dc.contributor.authorLukic, Z.
dc.contributor.authorGoff, S.
dc.contributor.authorCampbell, E.
dc.contributor.authorArriagada, G.
dc.date.accessioned2023-05-15T19:42:05Z
dc.date.available2023-05-15T19:42:05Z
dc.date.issued2013
dc.descriptionIndexación: Scopus.es
dc.description.abstractTRIM5α is a member of the tripartite motif family of proteins that restricts retroviral infection in a species-specific manner. The restriction requires an interaction between the viral capsid lattice and the B30.2/SPRY domain of TRIM5α. Previously, we determined that two SUMO interacting motifs (SIMs) present in the B30.2/SPRY domain of human TRIM5α (huTRIM5α) were important for the restriction of N-tropic Murine Leukemia Virus. Here, we examined whether SUMO expression and the SIM1 and SIM2 motifs in rhesus monkey TRIM5α (rhTRIM5α) are similarly important for Human Immunodeficiency Type 1 (HIV-) restriction. Results: We found that mutation of SIM1 and SIM2 of rhTRIM5α abolished the restriction of HIV-1 virus. Further, knockdown of SUMO-1 in rhTRIM5α expressing cells abolished restriction of HIV-1. These results may be due, in part, to the ability of SUMO-1 to stabilize rhTRIM5α protein expression, as SUMO-1 knockdown increased rhTRIM5α turnover and the mutations in SIM1 and SIM2 led to more rapid degradation than the wild type protein. The NF-κB signaling ability of rhTRIM5α was also attenuated by SUMO-1 knockdown. Finally, upon inhibition of CRM1-dependent nuclear export with Leptomycin B (LMB), wild type rhTRIM5α localized to SUMO-1 bodies in the nucleus, while the SIM1 and SIM2 mutants did not localize to SUMO-1. Conclusions: Our results suggest that the rhTRIM5α B30.2/SPRY domain is not only important for the recognition of the HIV-1 CA, but it is also important for its association with SUMO-1 or SUMO-1 modified proteins. These interactions help to maintain TRIM5α protein levels and its nuclear localization into specific nuclear bodies.es
dc.description.urihttps://retrovirology.biomedcentral.com/articles/10.1186/1742-4690-10-10
dc.identifier.citationRetrovirology, Volume 10, Issue 11, February 2013, Article number 10es
dc.identifier.doi10.1186/1742-4690-10-10
dc.identifier.issn1742-4690
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/49652
dc.language.isoenes
dc.publisherBMCes
dc.rights.licenseAttribution 2.0 Generic (CC BY 2.0)
dc.rights.urihttps://retrovirology.biomedcentral.com/articles/10.1186/1742-4690-10-10#rightslink
dc.subjectCapsides
dc.subjectVirus Capsides
dc.subjectPRY-SPRY Domaines
dc.subjectHuman immunodeficiency virus 1es
dc.subjectMacaca mulattaes
dc.subjectMiridaees
dc.subjectMurine leukemia viruses
dc.titleRole of SUMO-1 and SUMO interacting motifs in rhesus TRIM5α-mediated restrictiones
dc.typeArtículoes
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