Biochemical, serological, and genetic characterisation of Renibacterium salmoninarum isolates recovered from salmonids in Chile

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dc.contributor.authorBethke, J.
dc.contributor.authorQuezada, J.
dc.contributor.authorPoblete-Morales, M.
dc.contributor.authorIrgang, R.
dc.contributor.authorYáňez, A.
dc.date.accessioned2018-02-01T14:11:49Z
dc.date.available2018-02-01T14:11:49Z
dc.date.issued2017
dc.descriptionIndexación: Web of Science; Scopus.es_CL
dc.description.abstractThe Gram-positive Renibacterium salmoninarum causes bacterial kidney disease, a serious threat to Chilean salmon farming. To aid in vaccine development, this study used biochemical, antigenic, and genetic techniques to characterise 39 R. salmoninarum isolates from diseased Salmo salar and Oncorhynchus kisutch. Regardless of host, R. salmoninarum isolates were highly homogeneous pheno-typically. However, the isolates presented varied hydrophobicity (10-90%), as found by hydrocarbon adhesions, and distinct siderophores production, as evaluated on chrome azurol S agar. Serological assays established antigenic homogeneity among isolates, which can facilitate vaccine development. While western-blot profiles (using antiserum against iron-limited R. salmoninarum) differed, all isolates belonged to the same serogroup. Finally, RAPD, ERIC-PCR, and REP-PCR supported genetic R. salmoninarum homogeneity, suggesting clonal relationships. In conclusion, high homogeneity might facilitate vaccine development, but in vivo studies are needed to clarify the relationship of R. salmoninarum virulence with hydrophobicity/siderophores synthesis.es_CL
dc.description.urihttps://eafp.org/bulletin-archive/
dc.identifier.citationBulletin of the European Association of Fish Pathologists. Volume 37, Issue 4, 2017, Pages 169-180es_CL
dc.identifier.issn0108-0288
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/5230
dc.language.isoenes_CL
dc.publisherFRS Marine Laboratoryes_CL
dc.subjectCELL-SURFACE HYDROPHOBICITYes_CL
dc.subjectPOLYACRYLAMIDE-GELSes_CL
dc.subjectANTIGENIC VARIATIONes_CL
dc.subjectFISHes_CL
dc.subjectVIRULENCEes_CL
dc.subjectPROTEINSes_CL
dc.subjectBACTERIUMes_CL
dc.subjectSTRAINSes_CL
dc.subjectKIDNEYes_CL
dc.titleBiochemical, serological, and genetic characterisation of Renibacterium salmoninarum isolates recovered from salmonids in Chilees_CL
dc.typeArtículoes_CL
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