Expression of a novel non-coding mitochondrial RNA in human proliferating cells

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dc.contributor.authorVillegas, Jaime
dc.contributor.authorBurzio, Veronica
dc.contributor.authorVillota, Claudio
dc.contributor.authorLanderer, Eduardo
dc.contributor.authorMartinez, Ronny
dc.contributor.authorSantander, Marcela
dc.contributor.authorMartinez, Rodrigo
dc.contributor.authorPinto, Rodrigo
dc.contributor.authorVera, Maria I.
dc.contributor.authorBoccardo, Enrique
dc.contributor.authorVilla, Luisa L.
dc.contributor.authorBurzio, Luis O.
dc.date.accessioned2024-07-30T15:34:00Z
dc.date.available2024-07-30T15:34:00Z
dc.date.issued2007-12-01
dc.descriptionINDEXACIÓN: WEB OF SCIENCE.
dc.description.abstractPreviously, we reported the presence in mouse cells of a mitochondrial RNA which contains an inverted repeat (IR) of 121 nucleotides (nt) covalently linked to the 5 end of the mitochondrial 16S RNA (16S mtrRNA). Here, we report the structure of an equivalent transcript of 2374 nt which is over-expressed in human proliferating cells but not in resting cells. The transcript contains a hairpin structure comprising an IR of 815 nt linked to the 5 end of the 16S mtrRNA and forming a long double-stranded structure or stem and a loop of 40 nt. The stem is resistant to RNase A and can be detected and isolated after digestion with the enzyme. This novel transcript is a non-coding RNA (ncRNA) and several evidences suggest that the transcript is synthesized in mitochondria. The expression of this transcript can be induced in resting lymphocytes stimulated with phytohaemagglutinin (PHA). Moreover, aphidicolin treatment of DU145 cells reversibly blocks proliferation and expression of the transcript. If the drug is removed, the cells re-assume proliferation and over-express the ncmtRNA. These results suggest that the expression of the ncmtRNA correlates with the replicative state of the cell and it may play a role in cell proliferation.
dc.description.urifile:///C:/Users/montserrat.cornejo/Downloads/gkm863.pdf
dc.identifier.citationNUCLEIC ACIDS RESEARCH, Volume 35, Issue 21, Page 7336-7347
dc.identifier.doi10.1093/nar/gkm863
dc.identifier.issn0305-1048
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/58856
dc.language.isoen
dc.publisherOxford University Press
dc.rights.licenseAttribution-NonCommercial 2.0
dc.rights.uri(http://creativecommons.org/licenses/ by-nc/2.0/uk/
dc.subjectDOUBLE-STRANDED-RNA
dc.subjectRIBOSOMAL-RNA
dc.subjectREPLICATION SLIPPAGE
dc.subjectDELETION MUTAGENESIS
dc.subjectSECONDARY STRUCTURE
dc.subjectDNA-POLYMERASES
dc.subjectMESSENGER-RNA
dc.subjectHEAVY STRAND
dc.subjectHELA-CELLS
dc.subjectIN-VIVO
dc.titleExpression of a novel non-coding mitochondrial RNA in human proliferating cells
dc.typeArtículo
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