Molecular identification of Bartonella spp. and Rickettsia felis in fox fleas, Chile

Vista sencilla de ítem
dc.contributor.authorMillán, Javier
dc.contributor.authorSepúlveda-García, Paulina
dc.contributor.authorDi Cataldo, Sophia
dc.contributor.authorCanales, Nivia
dc.contributor.authorSallaberry-Pincheira, Nicole
dc.contributor.authorPainean, Javier
dc.contributor.authorCevidanes, Aitor
dc.contributor.authorMüller, Ananda
dc.date.accessioned2024-10-16T21:43:21Z
dc.date.available2024-10-16T21:43:21Z
dc.date.issued2023-05
dc.descriptionIndexación: Scopus
dc.description.abstractSeventy-five flea pools (one to ten fleas per pool) from 51 Andean foxes (Lycalopex culpaeus) and five South American grey foxes or chillas (Lycalopex griseus) from the Mediterranean region of Chile were analyzed for the presence of DNA of Bartonella spp. and Rickettsia spp. through quantitative real-time PCR for the nouG and gltA genes, respectively. Positive samples were further characterized by conventional PCR protocols, targeting gltA and ITS genes for Bartonella, and gltA, ompA, and ompB genes for Rickettsia. Bartonella was detected in 48 % of the Pulex irritans pools (B. rochalimae in three pools, B. berkhoffii in two pools, B. henselae in one pool), and 8 % of the Ctenocephalides felis felis pools (B. rochalimae, one pool). Rickettsia was confirmed in 11 % of P. irritans pools and 92 % of the Ct. felis pools. Characterization confirmed R. felis in all sequenced Rickettsia-positive pools. All Ct. canis pools were negative. A Ct. felis pool from a wild-found domestic ferret (Mustela putorius furo) also resulted positive for R. felis. Although opportunistic, this survey provides the first description of zoonotic pathogens naturally circulating in fleas parasitizing Chilean free-living carnivores. © 2023 The Authors
dc.description.urihttps://www-sciencedirect-com.recursosbiblioteca.unab.cl/science/article/pii/S0147957123000413?via%3Dihub
dc.identifier.citationComparative Immunology, Microbiology and Infectious Diseases. Volume 96. May 2023. Article number 101983
dc.identifier.doi10.1016/j.cimid.2023.101983
dc.identifier.issn0147-9571
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/61335
dc.language.isoen
dc.publisherElsevier Ltd
dc.rights.licenseCC BY 4.0 Attribution 4.0 International Deed
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectCulpeo
dc.subjectFlea-Borne
dc.subjectPseudalopex
dc.subjectSouth America
dc.subjectVector-borne
dc.titleMolecular identification of Bartonella spp. and Rickettsia felis in fox fleas, Chile
dc.typeArtículo
Archivos
Bloque original
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
Millan_Molecular_identification_of_Bartonella_spp._and_Rickettsia_2023.pdf
Tamaño:
2.21 MB
Formato:
Adobe Portable Document Format
Descripción:
TEXTO COMPLETO EN INGLES
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar
Colecciones
Fac.CV - Artículos de Revista