Expression and activity of the Calvin-Benson-Bassham cycle transcriptional regulator CbbR from Acidithiobacillus ferrooxidans in Ralstonia eutropha

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dc.contributor.authorEsparza, Mario
dc.contributor.authorJedlicki, Eugenia
dc.contributor.authorDopson, Mark
dc.contributor.authorHolmes, David S.
dc.date.accessioned2023-03-27T14:12:17Z
dc.date.available2023-03-27T14:12:17Z
dc.date.issued2015-08
dc.descriptionIndexación: Scopuses
dc.description.abstractAutotrophic fixation of carbon dioxide into cellular carbon occurs via several pathways but quantitatively, the Calvin-Benson-Bassham cycle is the most important. CbbR regulates the expression of the cbb genes involved in CO2 fixation via the Calvin-Benson-Bassham cycle in a number of autotrophic bacteria. A gene potentially encoding CbbR (cbbRAF) has been predicted in the genome of the chemolithoautotrophic, extreme acidophile Acidithiobacillus ferrooxidans. However, this microorganism is recalcitrant to genetic manipulation impeding the experimental validation of bioinformatic predictions. Two novel functional assays were devised to advance our understanding of cbbRAF function using the mutated facultative autotroph Ralstonia eutropha H14 ΔcbbR as a surrogate host to test gene function: (i) cbbRAF was expressed in R. eutropha and was able to complement ΔcbbR; and (ii) CbbRAF was able to regulate the in vivo activity of four A. ferrooxidans cbb operon promoters in R. eutropha. These results open up the use of R. eutropha as a surrogate host to explore cbbRAF activity. © FEMS 2015.es
dc.description.urihttps://academic-oup-com.recursosbiblioteca.unab.cl/femsle/article/362/15/fnv108/549585
dc.identifier.citationFEMS Microbiology Letters Volume 362, Issue 15August 2015 Article number fnv108es
dc.identifier.doi10.1093/femsle/fnv108
dc.identifier.issn0378-1097
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/47895
dc.language.isoenes
dc.publisherOxford University Presses
dc.rights.licenseAtribución 4.0 Internacional (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectAcidithiobacilluses
dc.subjectMicroorganismses
dc.subjectAcid Mine Drainagees
dc.titleExpression and activity of the Calvin-Benson-Bassham cycle transcriptional regulator CbbR from Acidithiobacillus ferrooxidans in Ralstonia eutrophaes
dc.typeArtículoes
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