11-Deoxycorticosterone (DOC)’s Action on the Gill Osmoregulation of Juvenile Rainbow Trout (Oncorhynchus mykiss)

Vista sencilla de ítem
dc.contributor.authorZuloaga, Rodrigo
dc.contributor.authorAhumada-Langer, Luciano
dc.contributor.authorAedo, Jorge Eduardo
dc.contributor.authorMolina, Alfredo
dc.contributor.authorValdés, Juan Antonio
dc.date.accessioned2024-04-19T16:29:15Z
dc.date.available2024-04-19T16:29:15Z
dc.date.issued2024-02-02
dc.descriptionIndexación: Scopus.
dc.description.abstractIn aquaculture, stress can negatively affect fish growth. For years, the cortisol hormone has been thought to play both glucocorticoid and mineralocorticoid functions. Nevertheless, recent research has suggested that 11-deoxycorticosterone (DOC) released during stress could contribute to cortisol actions, though this process is still misunderstood. Here, we evaluated the DOC effects on physiological and early transcriptional responses by RNA-seq. Juvenile rainbow trout were treated with DOC and/or glucocorticoids (mifepristone) or mineralocorticoid (eplerenone) receptor antagonists. Subsequently, plasma was collected, and cDNA libraries were generated from the gills of vehicle (control), DOC, mifepristone, mifepristone with DOC, eplerenone, and eplerenone with DOC groups. Calcium and phosphate levels in plasma were changed. Results revealed 914 differentially expressed transcripts (DETs) induced by DOC compared with control, mainly associated with sodium ion transmembrane transport, gluconeogenesis, negative regulation of transmembrane transport, and activation of innate immune response. DOC versus eplerenone with DOC comparison displayed 444 DETs related to cell-cell junction organization, canonical glycolysis, positive regulation of immune response, and potassium ion transport. Conversely, no DETs were detected in DOC versus mifepristone with DOC comparison. These data suggest that DOC has a relevant role in gill stress response and ion transport, which is differentially regulated by mineralocorticoid receptors.
dc.description.urihttps://www-scopus-com.recursosbiblioteca.unab.cl/record/display.uri?eid=2-s2.0-85190165032&origin=resultslist&sort=plf-f&src=s&nlo=&nlr=&nls=&sid=ebd6ea56a4cd106725879b6e21bf9121&sot=aff&sdt=cl&cluster=scofreetoread%2c%22all%22%2ct&sl=34&s=AF-ID%2860002636%29+AND+SUBJAREA%28AGRI%29&relpos=5&citeCnt=0&searchTerm=
dc.identifier.citationBiology Open Access Volume 13, Issue 2 February 2024 Article number 107
dc.identifier.doi10.3390/biology13020107
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/56139
dc.language.isoen
dc.publisherMDPI
dc.rights.licenseCC BY 4.0 DEED Attribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectDOC
dc.subjectgills
dc.subjectmineralocorticoid receptor
dc.subjectRNA-seq
dc.subjectsalmonid
dc.subjectstress response
dc.title11-Deoxycorticosterone (DOC)’s Action on the Gill Osmoregulation of Juvenile Rainbow Trout (Oncorhynchus mykiss)
Archivos
Bloque original
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
Zuloaga-R-11Deoxycorticosterone-DOCs-Action-on-the-Gill-Osmoregulation-of-Juvenile-Rainbow-Trout-Oncorhynchus-mykiss.pdf
Tamaño:
2.2 MB
Formato:
Adobe Portable Document Format
Descripción:
TEXTO COMPLETO EN INGLÉS
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar
Colecciones
Fac.CV - Artículos de Revista