Induction of a Specific Humoral Immune Response by Nasal Delivery of Bcla2ctd of Clostridioides difficile

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dc.contributor.authorMaia, Ana Raquel
dc.contributor.authorReyes-Ramírez, Rodrigo
dc.contributor.authorPizarro-Guajardo, Marjorie
dc.contributor.authorSaggese, Anella
dc.contributor.authorCastro-Córdova, Pablo
dc.contributor.authorIsticato, Rachele
dc.contributor.authorRicca, Ezio
dc.contributor.authorParedes-Sabja, Daniel
dc.contributor.authorBaccigalupi, Loredana
dc.date.accessioned2021-09-20T13:56:41Z
dc.date.available2021-09-20T13:56:41Z
dc.date.issued2020-02
dc.description.abstractClostridioides difficile, formerly known as Clostridium difficile, is a spore-forming bacterium considered as the most common cause of nosocomial infections in developed countries. The spore of C. difficile is involved in the transmission of the pathogen and in its first interaction with the host; therefore, a therapeutic approach able to control C. difficile spores would improve the clearance of the infection. The C-terminal (CTD) end of BclA2, a spore surface protein of C. difficile responsible of the interaction with the host intestinal cells, was selected as a putative mucosal antigen. The BclA2 fragment, BclA2CTD, was purified and used to nasally immunize mice both as a free protein and after adsorption to the spore of Bacillus subtilis, a well-established mucosal delivery vehicle. While the adsorption to spores increased the in vitro stability of BclA2CTD, in vivo both free and spore-adsorbed BclA2CTD were able to induce a similar, specific humoral immune response in a murine model. Although in the experimental conditions utilized the immune response was not protective, the induction of specific IgG indicates that free or spore-bound BclA2CTD could act as a putative mucosal antigen targeting C. difficile spores. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.es
dc.description.sponsorshipIndexación:Scopuses
dc.identifier.citationInternational Journal of Molecular SciencesOpen AccessVolume 21, Issue 42 February 2020 Article number 1277es
dc.identifier.issn16616596
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/20305
dc.language.isoenes
dc.publisherMDPI AGes
dc.subjectspore adsorption; spore antigens; Bacillus subtilis; spore surface display; mucosal vaccinationes
dc.titleInduction of a Specific Humoral Immune Response by Nasal Delivery of Bcla2ctd of Clostridioides difficilees
dc.typeArtículoes
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