The ompW (porin) gene mediates methyl viologen (paraquat) efflux in Salmonella enterica serovar Typhimurium

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dc.contributor.authorGil, F
dc.contributor.authorIpinza, F
dc.contributor.authorFuentes, J
dc.contributor.authorPumeron, P
dc.contributor.authorVillarreal, JM
dc.contributor.authorAspée, A
dc.contributor.authorMora, GC
dc.contributor.authorVásquez, CC
dc.contributor.authorSaavedra, C
dc.date.accessioned2024-07-06T14:52:16Z
dc.date.available2024-07-06T14:52:16Z
dc.date.issued2007-07-01
dc.descriptionINDEXACIÓN: WEB OF SCIENCE.
dc.description.abstractPorins are channels that enable passive diffusion of hydrophilic solutes, nutrients and toxins through the outer bacterial membrane. This explains in part the ability of Gram-negative microorganisms to grow in several different environments, as well as their drug resistance. OmpD is an outer membrane channel that works with the inner membrane pump YddG to expel methyl viologen (MV) from Salmonella enterica serovar Typhimurium: this occurs independently of SmvA, also involved in MV resistance. On the other hand, Delta tolC strains show increased MV resistance when compared to wild-type cells, suggesting that there may be other porin(s) that could function with SmvA to pump MV out of S. typhimurium. A strong candidate is OmpW. Here we show that Delta ompW strains of S. typhimurium are 2.5-fold more sensitive to MV than the wild-type strain. Transcriptional fusions replacing ompW by lacZ show that ompW is induced at least 2-fold in the presence of MV. This result was observed both at the mRNA and protein levels, suggesting that ompW participates in MV resistance. In addition, Delta smvA Delta ompW strains are not fully complemented by smvA, suggesting that OmpW may function through an independent pathway different from that used by SmvA to move MV outside the cell. (C) 2007 Elsevier Masson SAS. All rights reserved.
dc.identifier.citationRESEARCH IN MICROBIOLOGY, Volume 158, Issue 6, Page 529-536
dc.identifier.doi10.1016/j.resmic.2007.05.004
dc.identifier.issn0923-2508
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/58250
dc.language.isoen
dc.publisherElsevier
dc.rights.licenseCC BY-NC-ND 4.0 ATRIBUCIÓN-NOCOMERCIAL-SINDERIVADAS 4.0 INTERNACIONAL Deed
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subjectporin
dc.subjectSalmonella typhimurium
dc.subjectOmpW
dc.subjectmethyl viologen
dc.titleThe ompW (porin) gene mediates methyl viologen (paraquat) efflux in Salmonella enterica serovar Typhimurium
dc.typeArtículo
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