Production and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen Piscirickettsia salmonis

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dc.contributor.authorWilhelm, Vivian
dc.contributor.authorSoza, Cristian
dc.contributor.authorMartinez, Rodrigo
dc.contributor.authorRosemblatt, Mario
dc.contributor.authorBurzio, Luis
dc.contributor.authorValenzuela, Pablo D.T.
dc.date.accessioned2014-07-02T20:46:43Z
dc.date.accessioned2016-05-24T18:45:39Z
dc.date.available2014-07-02T20:46:43Z
dc.date.available2016-05-24T18:45:39Z
dc.date.issued2005
dc.descriptionhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100009&lng=es&nrm=isoes
dc.description.abstractWe have isolated and sequenced the genes encoding the heat shock proteins 60 (Hsp60) and 70 (Hsp70) of the salmon pathogen Piscirickettsia salmonis. The sequence analysis revealed the expected two open reading frames that encode proteins with calculated molecular weights of 60,060 and 70,400. The proteins exhibit a 70-80% homology with other known prokaryotic Hsp60 and Hsp70 sequences. The coding regions have been expressed in E. coli as thioredoxin fusion proteins. Both recombinant proteins were shown to elicit a humoral response when injected intraperitoneally in Atlantic salmon and also conferred protection to fish challenged with P. salmonis. The present data will facilitate further studies on the involvement of heat shock proteins in protective immunity of fish to infection by P. salmonis and their potential use in recombinants vaccines against this intracellular pathogen.es
dc.identifier.citationBiol. Res. v.38 n.1 Santiago 2005es
dc.identifier.issn0716-9760
dc.identifier.otherDOI: http://dx.doi.org/10.4067/S0716-97602005000100009
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/2477
dc.language.isoenes
dc.publisherSociedad de Biología de Chilees
dc.subjectPiscirickettsia salmonises
dc.subjectHsp60es
dc.subjectHsp70es
dc.subjectgroELes
dc.subjectdnaKes
dc.subjectimmune responsees
dc.titleProduction and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen Piscirickettsia salmonises
dc.typeArtículoes
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