Lipopolysaccharide inhibits the channel activity of the P2X7 receptor

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dc.contributor.authorLeiva-Salcedo, E.
dc.contributor.authorCoddou, C.
dc.contributor.authorRodríguez, F.
dc.contributor.authorPenna, A.
dc.contributor.authorLopez, X.
dc.contributor.authorNeira, T.
dc.contributor.authorFernández, R.
dc.contributor.authorImarai, M.
dc.contributor.authorRios, M.
dc.contributor.authorEscobar, J.
dc.contributor.authorMontoya, M.
dc.contributor.authorHuidobro-Toro, J.
dc.contributor.authorEscobar, A.
dc.contributor.authorAcuña-Castillo, C.
dc.date.accessioned2023-07-31T17:02:10Z
dc.date.available2023-07-31T17:02:10Z
dc.date.issued2011
dc.descriptionIndexación: Scopus.es
dc.description.abstractThe purinergic P2X7 receptor (P2X7R) plays an important role during the immune response, participating in several events such as cytokine release, apoptosis, and necrosis. The bacterial endotoxin lipopolysaccharide (LPS) is one of the strongest stimuli of the immune response, and it has been shown that P2X7R activation can modulate LPS-induced responses. Moreover, a C-terminal binding site for LPS has been proposed. In order to evaluate if LPS can directly modulate the activity of the P2X7R, we tested several signaling pathways associated with P2X7R activation in HEK293 cells that do not express the TLR-4 receptor. We found that LPS alone was unable to induce any P2X7R-related activity, suggesting that the P2X7R is not directly activated by the endotoxin. On the other hand, preapplication of LPS inhibited ATP-induced currents, intracellular calcium increase, and ethidium bromide uptake and had no effect on ERK activation in HEK293 cells. In splenocytes-derived T-regulatory cells, in which ATP-induced apoptosis is driven by the P2X7R, LPS inhibited ATP-induced apoptosis. Altogether, these results demonstrate that LPS modulates the activity of the P2X7R and suggest that this effect could be of physiological relevance.es
dc.description.urihttps://www.hindawi.com/journals/mi/2011/152625/
dc.identifier.citationMediators of Inflammation, Volume 2011, 2011, Article number 152625es
dc.identifier.doi10.1155/2011/152625
dc.identifier.issn1466-1861
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/52107
dc.language.isoenes
dc.rights.licenseAttribution 3.0 Unported (CC BY 3.0)
dc.rights.urihttps://www.hindawi.com/journals/mi/2011/152625/#copyright
dc.subjectPurinergic P2X7 Receptorses
dc.subjectAnimalses
dc.subjectAz10606120es
dc.subjectpriority journales
dc.subjectregulatory T lymphocytees
dc.subjectsignal transductiones
dc.subjectspleen celles
dc.titleLipopolysaccharide inhibits the channel activity of the P2X7 receptores
dc.typeArtículoes
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