Salmonella Typhimurium exhibits fluoroquinolone resistance mediated by the accumulation of the antioxidant molecule H2S in a CysK-dependent manner

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dc.contributor.authorFrávega, J.
dc.contributor.authorÁlvarez, R.
dc.contributor.authorDíaz, F.
dc.contributor.authorInostroza, O.
dc.contributor.authorTejías, C.
dc.contributor.authorRodas, P.I.
dc.contributor.authorParedes-Sabja, D.
dc.contributor.authorFuentes, J.A.
dc.contributor.authorCalderón, I.L.
dc.contributor.authorGil, F.
dc.date.accessioned2023-11-22T18:18:32Z
dc.date.available2023-11-22T18:18:32Z
dc.date.issued2016
dc.descriptionIndexación: Scopus.es
dc.description.abstractObjectives To evaluate the contribution of cysK and cysM to the fluoroquinolone (ofloxacin) antibiotic resistance in Salmonella Typhimurium, and their impact on H2S and cysteine production through targeted mutagenesis. Methods Salmonella Typhimurium 14028s and its cysK and cysM mutants were tested for their susceptibility to ofloxacin, as determined by a broth microdilution test (to determine the MIC) and survival curves. H2S levels were measured by the Pb(AC)2 method and cysteine levels were determined using 5,5-dithio-bis-2-nitrobenzoic acid. DNA damage induced by antibiotic treatment was determined by PFGE. Finally, expression of cysK and cysM genes under antibiotic treatment was determined by real-time reverse transcription PCR. Results As determined by MIC, the ΔcysK strain was more resistant to ofloxacin, a reactive oxygen species (ROS)-producing fluoroquinolone, than the WT and ΔcysM strains, which correlates with survival curves. Moreover, the ΔcysK strain exhibited higher H2S levels and lower cysteine levels than the WT strain. Finally, the ΔcysK strain exhibited lower DNA damage upon challenge with ofloxacin than the WT and ΔcysM strains. These results are in accordance with lower expression of cysK under ofloxacin treatment in the WT strain. Conclusions This work demonstrated that cysteine metabolism in Salmonella Typhimurium modulated H2S levels, conferring resistance to second-generation fluoroquinolones.es
dc.description.urihttps://academic-oup-com.recursosbiblioteca.unab.cl/jac/article/71/12/3409/2631268
dc.identifier.citationJournal of Antimicrobial Chemotherapy. Volume 71, Issue 12, Pages 3409 - 3415. 2016es
dc.identifier.doi10.1093/JAC/DKW311
dc.identifier.issn0305-7453
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/54035
dc.language.isoenes
dc.publisherOxford University Presses
dc.subjectAntibioticses
dc.subjectAntioxidantses
dc.subjectPolymerase Chain Reactiones
dc.subjectMetabolismes
dc.subjectAntibiotic Resistancees
dc.subjectBacteriales
dc.subjectCysteinees
dc.subjectDna Damagees
dc.subjectElectrophoresises
dc.subjectGeles
dc.subjectPulsed-fieldes
dc.subjectFluoroquinoloneses
dc.subjectGeneses
dc.subjectOfloxacines
dc.subjectReactive Oxygen Specieses
dc.subjectSalmonella Typhimuriumes
dc.subjectSurvival Curvees
dc.subjectMulti-Antibiotic resistancees
dc.subjectMoleculees
dc.titleSalmonella Typhimurium exhibits fluoroquinolone resistance mediated by the accumulation of the antioxidant molecule H2S in a CysK-dependent manneres
dc.typeArtículoes
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