Multi Locus Sequence Typing scheme for Acidithiobacillus caldus strain evaluation and differentiation

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dc.contributor.authorNuñez, Harold
dc.contributor.authorLoyola, David
dc.contributor.authorCárdenas, Juan Pablo
dc.contributor.authorHolmes, David S.
dc.contributor.authorJohnson, D. Barrie
dc.contributor.authorQuatrini, Raquel
dc.date.accessioned2023-01-03T15:13:57Z
dc.date.available2023-01-03T15:13:57Z
dc.date.issued2014
dc.descriptionIndexación: Scopuses
dc.description.abstractPhenotypic, metabolic and genetic properties of several Acidithiobacillus caldus strains indicate the existence of as yet undefined levels of variation within the species. Inspite of this, intraspecies genetic diversity has not yet been explored in detail. In this study, the design and implementation of a Multi Locus Sequence Typing (MLST) scheme for At. caldus is described. This represents the first MLST-based study applied to industrial isolates of the species. Seven informative and discriminant MLST markers were selected using a sequence-driven approach and a custom-designed bioinformatic pipeline. The allelic profiles of thirteen At. caldus strains from diverse geographical origins and industrial settings were derived using this scheme. MLST-based population structure analysis indicated only moderate amounts of genetic diversity within the set of strains, further supporting their current assignment to a single species. Also, no clear evidence for geographical isolation could be derived from this study. However, the prevalence of sequence type 1 in heap leaching industrial settings support the view that bioprocess conditions and dynamics may have a strong influence on At. caldus (microbial) microdiversity patterns. The MLST scheme presented herein is a valuable tool for the identification and classification of strains of At. caldus for either ecological or evolutionary studies and possibly also for industrial monitoring purposes. © 2014 The Authors.es
dc.description.urihttps://www-sciencedirect-com.recursosbiblioteca.unab.cl/science/article/pii/S0923250814001168?via%3Dihub
dc.identifier.doi10.1016/j.resmic.2014.07.014
dc.identifier.issn0923-2508
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/35762
dc.language.isoenes
dc.publisherElsevier Masson s.r.l.es
dc.rights.licenseAtribución 4.0 Internacional (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectAcidithiobacillus calduses
dc.subjectIndustrial isolateses
dc.subjectMulti Locus Sequence Typing (MLST)es
dc.subjectPhylogeneticses
dc.subjectSequence typeses
dc.subjectSNPses
dc.titleMulti Locus Sequence Typing scheme for Acidithiobacillus caldus strain evaluation and differentiationes
dc.typeArtículoes
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Research in Microbiology Volume 165, Issue 9, Pages 735 - 7421 November 2014
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