Sex-Dependent Changes of miRNA Levels in the Hippocampus of Adrenalectomized Rats following Acute Corticosterone Administration

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dc.contributor.authorCorrales, Wladimir A.
dc.contributor.authorSilva, Juan P.
dc.contributor.authorParra, Claudio S.
dc.contributor.authorOlave, Felipe A.
dc.contributor.authorAguayo, Felipe I.
dc.contributor.authorRomán-Albasini, Luciano
dc.contributor.authorAliaga, Esteban
dc.contributor.authorVenegas-Zamora, Leslye
dc.contributor.authorAvalos, Ana M.
dc.contributor.authorRojas, Paulina S.
dc.contributor.authorMaracaja-Coutinho, Vinicius
dc.contributor.authorOakley, Robert H.
dc.contributor.authorCidlowski, John A.
dc.contributor.authorFiedler, Jenny L.
dc.date.accessioned2025-06-10T16:28:08Z
dc.date.available2025-06-10T16:28:08Z
dc.date.issued2021-08-18
dc.descriptionIndexación: Scopus.
dc.description.abstractWe explored sex-biased effects of the primary stress glucocorticoid hormone corticosterone on the miRNA expression profile in the rat hippocampus. Adult adrenalectomized (ADX) female and male rats received a single corticosterone (10 mg/kg) or vehicle injection, and after 6 h, hippocampi were collected for miRNA, mRNA, and Western blot analyses. miRNA profiling microarrays showed a basal sex-biased miRNA profile in ADX rat hippocampi. Additionally, acute corticosterone administration triggered a sex-biased differential expression of miRNAs derived from genes located in several chromosomes and clusters on the X and 6 chromosomes. Putative promoter analysis unveiled that most corticosterone-responsive miRNA genes contained motifs for either direct or indirect glucocorticoid actions in both sexes. The evaluation of transcription factors indicated that almost 50% of miRNA genes sensitive to corticosterone in both sexes was under glucocorticoid receptor regulation. Transcription factor-miRNA regulatory network analyses identified several transcription factors that regulate, activate, or repress miRNA expression. Validated target mRNA analysis of corticosterone-responsive miRNAs showed a more complex miRNA-mRNA interaction network in males compared to females. Enrichment analysis revealed that several hippocampal-relevant pathways were affected in both sexes, such as neurogenesis and neurotrophin signaling. The evaluation of selected miRNA targets from these pathways displayed a strong sex difference in the hippocampus of ADX-vehicle rats. Corticosterone treatment did not change the levels of the miRNA targets and their corresponding tested proteins. Our data indicate that corticosterone exerts a sex-biased effect on hippocampal miRNA expression, which may engage in sculpting the basal sex differences observed at higher levels of hippocampal functioning. © 2021 American Chemical Society.
dc.description.accesoabiertoSI
dc.description.agradThis work was supported by the Intramural Research Program of the NIEHS, National Institutes of Health (J.A.C.) and FONDECYT 1190899 (J.L.F.).
dc.description.urihttps://pubs-acs-org.recursosbiblioteca.unab.cl/doi/10.1021/acschemneuro.0c00762
dc.identifier.citationACS Chemical Neuroscience, Volume 12, Issue 16, Pages 2981 - 3001, 18 August 2021
dc.identifier.doi10.1021/acschemneuro.0c00762
dc.identifier.folioFONDECYT 1190899 (J.L.F.).
dc.identifier.generoH
dc.identifier.issn1948-7193
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/65161
dc.language.isoen
dc.other.orcidhttps://orcid.org/0000-0002-4176-0116
dc.other.orcidhttps://orcid.org/0000-0002-9106-598X
dc.other.orcidhttps://orcid.org/0000-0002-7523-5635
dc.other.orcidhttps://orcid.org/0000-0002-8873-9381
dc.publisherAmerican Chemical Society
dc.rights.licenseAtribución/Reconocimiento 4.0 Internacional CC BY 4.0 Deed
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectAdrenalectomy
dc.subjectCorticosterone
dc.subjectGlucocorticoid receptors
dc.subjectHippocampus
dc.subjectmiRNAs
dc.subjectSex bias
dc.titleSex-Dependent Changes of miRNA Levels in the Hippocampus of Adrenalectomized Rats following Acute Corticosterone Administration
dc.typeArtículo
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