Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes

dc.contributor.authorDhonukshe, Pankaj
dc.contributor.authorGrigoriev, Ilya
dc.contributor.authorFischer, Rainer
dc.contributor.authorTominaga, Motoki
dc.contributor.authorRobinson, David G.
dc.contributor.authorHasek, Jiri
dc.contributor.authorPaciorek, Tomasz
dc.contributor.authorPetrasek, Jan
dc.contributor.authorSeifertova, Daniela
dc.contributor.authorTejos, Ricardo
dc.contributor.authorMeisel, Lee A.
dc.contributor.authorZazimalova, Eva
dc.contributor.authorGadella, Theodorus W. J., Jr.
dc.contributor.authorStierhof, York-Dieter
dc.contributor.authorUeda, Takashi
dc.contributor.authorOiwa, Kazuhiro
dc.contributor.authorAkhmanova, Anna
dc.contributor.authorBrock, Roland
dc.contributor.authorSpang, Anne
dc.contributor.authorFriml, Jiri
dc.date.accessioned2024-07-30T16:16:10Z
dc.date.available2024-07-30T16:16:10Z
dc.date.issued2008-03-18
dc.descriptionINDEXACIÓN: WEB OF SCIENCE.
dc.description.abstractMany aspects of plant development, including patterning and tropisms, are largely dependent on the asymmetric distribution of the plant signaling molecule auxin. Auxin transport inhibitors (ATIs), which interfere with directional auxin transport, have been essential tools in formulating this concept. However, despite the use of ATIs in plant research for many decades, the mechanism of ATI action has remained largely elusive. Using real-time live-cell microscopy, we show here that prominent ATIs such as 2,3,5-triiodobenzoic acid (TIBA) and 2-(1-pyrenoyl) benzoic acid (PBA) inhibit vesicle trafficking in plant, yeast, and mammalian cells. Effects on micropinocytosis, rab5-labeled endosomal motility at the periphery of HeLa cells and on fibroblast mobility indicate that ATIs influence actin cytoskeleton. Visualization of actin cytoskeleton dynamics in plants, yeast, and mammalian cells show that ATIs stabilize actin. Conversely, stabilizing actin by chemical or genetic means interferes with endocytosis, vesicle motility, auxin transport, and plant development, including auxin transport-dependent processes. Our results show that a class of ATIs act as actin stabilizers and advocate that actin-dependent trafficking of auxin transport components participates in the mechanism of auxin transport. These studies also provide an example of how the common eukaryotic process of actin-based vesicle motility can fulfill a plant-specific physiological role.
dc.description.urihttps://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC2393819&blobtype=pdf
dc.identifier.citationPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Volume 105, Issue 11, Page 4489-4494
dc.identifier.doi10.1073/pnas.0711414105
dc.identifier.issn0027-8424
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/58858
dc.language.isoen
dc.publisherNational Academy of Sciences
dc.subjectARABIDOPSIS-THALIANA
dc.subjectPLANT DEVELOPMENT
dc.subjectEFFLUX CARRIERS
dc.subjectEARLY ENDOSOMES
dc.subjectENDOCYTOSIS
dc.subjectPROTEIN
dc.subjectCELLS
dc.subjectROOT
dc.subjectORGANIZATION
dc.subjectNETWORK
dc.titleAuxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes
dc.typeArtículo
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