Microarray analysis of the Escherichia coli response to CdTe-GSH Quantum Dots: Understanding the bacterial toxicity of semiconductor nanoparticles

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dc.contributor.authorMonrás, Juan P.
dc.contributor.authorCollao, Bernardo
dc.contributor.authorMolina-Quiroz, Roberto C.
dc.contributor.authorPradenas, Gonzalo A.
dc.contributor.authorSaona, Luis A.
dc.contributor.authorDurán-Toro, Vicente
dc.contributor.authorÓrdenes-Aenishanslins, Nicolás
dc.contributor.authorVenegas, Felipe A.
dc.contributor.authorLoyola, David E.
dc.contributor.authorBravo, Denisse
dc.contributor.authorCalderón, Paulina F.
dc.contributor.authorCalderón, Iván L.
dc.contributor.authorVásquez, Claudio C.
dc.contributor.authorChasteen, Thomas G.
dc.contributor.authorLopez, Desiré A.
dc.contributor.authorPérez-Donoso, José M.
dc.date.accessioned2022-12-27T15:22:31Z
dc.date.available2022-12-27T15:22:31Z
dc.date.issued2014
dc.descriptionIndexación: Scopuses
dc.description.abstractBackground: Most semiconductor nanoparticles used in biomedical applications are made of heavy metals and involve synthetic methods that require organic solvents and high temperatures. This issue makes the development of water-soluble nanoparticles with lower toxicity a major topic of interest. In a previous work our group described a biomimetic method for the aqueous synthesis of CdTe-GSH Quantum Dots (QDs) using biomolecules present in cells as reducing and stabilizing agents. This protocol produces nanoparticles with good fluorescent properties and less toxicity than those synthesized by regular chemical methods. Nevertheless, biomimetic CdTe-GSH nanoparticles still display some toxicity, so it is important to know in detail the effects of these semiconductor nanoparticles on cells, their levels of toxicity and the strategies that cells develop to overcome it. Results: In this work, the response of E. coli exposed to different sized-CdTe-GSH QDs synthesized by a biomimetic protocol was evaluated through transcriptomic, biochemical, microbiological and genetic approaches. It was determined that: i) red QDs (5 nm) display higher toxicity than green (3 nm), ii) QDs mainly induce expression of genes involved with Cd+2 stress (zntA and znuA) and tellurium does not contribute significantly to QDs-mediated toxicity since cells incorporate low levels of Te, iii) red QDs also induce genes related to oxidative stress response and membrane proteins, iv) Cd2+ release is higher in red QDs, and v) QDs render the cells more sensitive to polymyxin B. Conclusion: Based on the results obtained in this work, a general model of CdTe-GSH QDs toxicity in E. coli is proposed. Results indicate that bacterial toxicity of QDs is mainly associated with cadmium release, oxidative stress and loss of membrane integrity. The higher toxicity of red QDs is most probably due to higher cadmium content and release from the nanoparticle as compared to green QDs. Moreover, QDs-treated cells become more sensitive to polymyxin B making these biomimetic QDs candidates for adjuvant therapies against bacterial infections. © 2014 Monrás et al.es
dc.description.urihttps://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-15-1099
dc.identifier.citationBMC Genomics Volume 15, Issue 1December 12, 2014 Article number 1099es
dc.identifier.doi10.1186/1471-2164-15-1099
dc.identifier.issn1471-2164
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/25559
dc.language.isoenes
dc.publisherBioMed Central Ltd.es
dc.rights.licenseAtribución 4.0 Internacional (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectCadmiumes
dc.subjectNanoparticleses
dc.subjectOxidative stresses
dc.subjectToxicity mechanismes
dc.subjectTranscriptomic responsees
dc.titleMicroarray analysis of the Escherichia coli response to CdTe-GSH Quantum Dots: Understanding the bacterial toxicity of semiconductor nanoparticleses
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