Bioinformatic prediction and experimental verification of Fur-regulated genes in the extreme acidophile Acidithiobacillus ferrooxidans

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dc.contributor.authorQuatrini, Raquel
dc.contributor.authorLefimil, Claudia
dc.contributor.authorVeloso, Felipe A.
dc.contributor.authorPedroso, Inti
dc.contributor.authorHolmes, David S.
dc.contributor.authorJedlicki, Eugenia
dc.date.accessioned2024-04-01T17:12:38Z
dc.date.available2024-04-01T17:12:38Z
dc.date.issued2007-04
dc.descriptionINDEXACIÓN: SCOPUS.
dc.description.abstractThe γ-proteobacterium Acidithiobacillus ferrooxidans lives in extremely acidic conditions (pH 2) and, unlike most organisms, is confronted with an abundant supply of soluble iron. It is also unusual in that it oxidizes iron as an energy source. Consequently, it faces the challenging dual problems of (i) maintaining intracellular iron homeostasis when confronted with extremely high environmental loads of iron and (ii) of regulating the use of iron both as an energy source and as a metabolic micronutrient. A combined bioinformatic and experimental approach was undertaken to identify Fur regulatory sites in the genome of A. ferrooxidans and to gain insight into the constitution of its Fur regulon. Fur regulatory targets associated with a variety of cellular functions including metal trafficking (e.g. feoPABC, tdr, tonBexbBD, copB, cdf), utilization (e.g. fdx, nif, transcriptional regulation (e.g. phoB, irr, iscR) and redox balance (grx), trx, gst) were identified. Selected predicted Fur regulatory sites were confirmed by FURTA, EMSA and in vitro transcription analyses. This study provides the first model for a Fur-binding site consensus sequence in an acidophilic iron-oxidizing microorganism and lays the foundation for future studies aimed at deepening our understanding of the regulatory networks that control iron uptake, homeostasis and oxidation in extreme acidophiles. © 2007 The Author(s).
dc.identifier.citationNucleic Acids Research, Volume 35, Issue 7, Pages 2153 - 2166, April 2007
dc.identifier.doi10.1093/nar/gkm068
dc.identifier.issn1362-4962
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/55514
dc.language.isoen
dc.rights.licenseCC BY 4.0 DEED Atribución 4.0 Internacional
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectAcidithiobacillus
dc.subjectBacterial Proteins
dc.subjectBase Sequence
dc.subjectBinding Sites
dc.subjectComputational Biology
dc.subjectConsensus Sequence
dc.subjectGene Expression Regulation, Bacterial
dc.subjectGenomics
dc.subjectIron
dc.subjectPromoter Regions (Genetics)
dc.subjectRegulatory Elements, Transcriptional
dc.subjectRegulon
dc.subjectRepressor Proteins
dc.subjectTranscription, Genetic
dc.titleBioinformatic prediction and experimental verification of Fur-regulated genes in the extreme acidophile Acidithiobacillus ferrooxidans
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