Antiviral Activity of an Endogenous Parvoviral Element

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dc.contributor.authorBravo, Angelica
dc.contributor.authorFernández-García, Leandro
dc.contributor.authorIbarra-Karmy, Rodrigo
dc.contributor.authorMardones, Gonzalo A.
dc.contributor.authorMercado, Luis
dc.contributor.authorBustos, Fernando J.
dc.contributor.authorGifford, Robert J.
dc.contributor.authorArriagada, Gloria
dc.date.accessioned2023-09-06T22:59:01Z
dc.date.available2023-09-06T22:59:01Z
dc.date.issued2023-07
dc.descriptionIndexación: Scopuses
dc.description.abstractEndogenous viral elements (EVEs) are genomic DNA sequences derived from viruses. Some EVEs have open reading frames (ORFs) that can express proteins with physiological roles in their host. Furthermore, some EVEs exhibit a protective role against exogenous viral infection in their host. Endogenous parvoviral elements (EPVs) are highly represented in mammalian genomes, and although some of them contain ORFs, their function is unknown. We have shown that the locus EPV-Dependo.43-ODegus, an EPV with an intact ORF, is transcribed in Octodon degus (degu). Here we examine the antiviral activity of the protein encoded in this EPV, named DeRep. DeRep was produced in bacteria and used to generate antibodies that recognize DeRep in western blots of degu tissue. To test if DeRep could protect against exogenous parvovirus, we challenged cells with the minute virus of mice (MVM), a model autonomous parvovirus. We observed that MVM protein expression, DNA damage induced by replication, viral DNA, and cytopathic effects are reduced when DeRep is expressed in cells. The results of this study demonstrate that DeRep is expressed in degu and can inhibit parvovirus replication. This is the first time that an EPV has been shown to have antiviral activity against an exogenous virus. © 2023 by the authors.es
dc.description.urihttps://www.mdpi.com/1999-4915/15/7/1420
dc.identifier.citationViruses Volume 15, Issue 7July 2023 Article number 1420es
dc.identifier.doi10.3390/v15071420
dc.identifier.issn1999-4915
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/53083
dc.language.isoenes
dc.publisherMDPIes
dc.rights.licenseAtribución 4.0 Internacional (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectEndogenous viral elementses
dc.subjectEvolutiones
dc.subjectImmunityes
dc.subjectPaleovirologyes
dc.subjectParvoviruses
dc.titleAntiviral Activity of an Endogenous Parvoviral Elementes
dc.typeArtículoes
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