Bioinspired Heparin Nanosponge Prepared by Photo-crosslinking for Controlled Release of Growth Factors

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dc.contributor.authorChoi, W.I.
dc.contributor.authorSahu, A.
dc.contributor.authorVilos, C.
dc.contributor.authorKamaly, N.
dc.contributor.authorJo, S.-M.
dc.contributor.authorLee, J.H.
dc.contributor.authorTae, G.
dc.date.accessioned2018-04-27T18:44:09Z
dc.date.available2018-04-27T18:44:09Z
dc.date.issued2017-06
dc.descriptionIndexación: Scopus.es_ES
dc.description.abstractGrowth factors have great therapeutic potential for various disease therapy and tissue engineering applications. However, their clinical efficacy is hampered by low bioavailability, rapid degradation in vivo and non-specific biodistribution. Nanoparticle based delivery systems are being evaluated to overcome these limitations. Herein, we have developed a thermosensitive heparin nanosponge (Hep-NS) by a one step photopolymerization reaction between diacrylated pluronic and thiolated heparin molecules. The amount of heparin in Hep-NS was precisely controlled by varying the heparin amount in the reaction feed. Hep-NS with varying amounts of heparin showed similar size and shape properties, though surface charge decreased with an increase in the amount of heparin conjugation. The anticoagulant activity of the Hep-NS decreased by 65% compared to free heparin, however the Hep-NS retained their growth factor binding ability. Four different growth factors, bFGF, VEGF, BMP-2, and HGF were successfully encapsulated into Hep-NS. In vitro studies showed sustained release of all the growth factors for almost 60 days and the rate of release was directly dependent on the amount of heparin in Hep-NS. The released growth factors retained their bioactivity as assessed by a cell proliferation assay. This heparin nanosponge is therefore a promising nanocarrier for the loading and controlled release of growth factors.es_ES
dc.description.urihttps://www.nature.com/articles/s41598-017-14040-5.pdf
dc.identifier.citationScientific Reports, 7(1), art. no. 14351.es_ES
dc.identifier.issn2045-2322
dc.identifier.otherDOI: 10.1038/s41598-017-14040-5
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/5684
dc.language.isoeses_ES
dc.publisherNature Publishing Groupes_ES
dc.titleBioinspired Heparin Nanosponge Prepared by Photo-crosslinking for Controlled Release of Growth Factorses_ES
dc.typeArtículoes_ES
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