Sweetening K-channels: what sugar taught us about permeation and gating

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dc.contributor.authorNaranjo, David
dc.contributor.authorDiaz-Franulic, Ignacio
dc.date.accessioned2024-04-05T18:40:10Z
dc.date.available2024-04-05T18:40:10Z
dc.date.issued2023
dc.descriptionIndexación: Scopus.
dc.description.abstractBecause they enable for the modification of both viscosity and osmolarity, sugars have been used as a biophysical probe of voltage-gated K-channels for a while. Viscosity variations made it possible to measure the pore sizes in large and small conductance K-channels using techniques similar to those used in the 1980s to study the gramicidin A channel. These analyses led to the finding that the size of the internal mouth appears to be the primary cause of the conductance differences between Shaker-like channels and large conductance BK-channels. As an osmotic agent, adding sugar unilaterally causes streaming potentials that indicate H2O/K+ cotransport across the BK-channel pore. Osmotic experiments on Shaker K-channels suggest that the pore gate operation and the slow inactivation displace comparable amounts of water. Functionally isolated voltage sensors allow estimation of individual osmotic work for each voltage sensing charge during voltage-activation, reporting dramatic internal and external remodeling of the Voltage Sensing Domain´s solvent exposed surfaces. Remarkably, each charge of the VSD appears to take a unique trajectory. Thus, manipulation of viscosity and osmolarity, together with 3D structures, brings in solid grounds to harmonize function and structure in membrane proteins such as K-channels and, in a wider scope, other structurally dynamic proteins. Copyright © 2023 Naranjo and Diaz-Franulic.
dc.description.urihttps://www.frontiersin.org/articles/10.3389/fmolb.2023.1063796/full
dc.identifier.citationFrontiers in Molecular Biosciences. Volume 10. 2023. Article number 1063796
dc.identifier.doi10.3389/fmolb.2023.1063796
dc.identifier.issn2296-889X
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/55703
dc.language.isoen
dc.publisherFrontiers Media S.A.
dc.rights.licensehttps://creativecommons.org/licenses/by/4.0/
dc.rights.uriCC BY 4.0 DEED Attribution 4.0 International
dc.subjectDiffusion limited current
dc.subjectKv-channels
dc.subjectOsmotic agent
dc.subjectSingle channel conductance
dc.subjectStreaming potential
dc.subjectVoltage gated transition
dc.subjectVolumetric work
dc.titleSweetening K-channels: what sugar taught us about permeation and gating
dc.typeArtículo
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