ATP Induces IL-1𝛽 Secretion in Neisseria gonorrhoeae-Infected Human Macrophages by a Mechanism Not Related to the NLRP3/ASC/Caspase-1 Axis

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dc.contributor.authorGarcia, K
dc.contributor.authorEscobar, G
dc.contributor.authorMendoza, P
dc.contributor.authorBeltran, C
dc.contributor.authorPerez, C
dc.contributor.authorArancibia, S
dc.contributor.authorVernal, R
dc.contributor.authorRodas, PI
dc.contributor.authorAcuna-Castillo, C
dc.contributor.authorEscobar, A
dc.date.accessioned2017-04-27T18:50:20Z
dc.date.available2017-04-27T18:50:20Z
dc.date.issued2016
dc.descriptionIndexación: Web of Science y Scopuses
dc.description.abstractNeisseria gonorrhoeae (Ngo) has developed multiple immune evasion mechanisms involving the innate and adaptive immune responses. Recent findings have reported that Ngo reduces the IL-1 beta secretion of infected human monocyte-derived macrophages (MDM). Here, we investigate the role of adenosine triphosphate (ATP) in production and release of IL-1 beta in Ngo-infected MDM. We found that the exposure of Ngo-infected MDM to ATP increases IL-1 beta levels about ten times compared with unexposed Ngo-infected MDM (P < 0.01). However, we did not observe any changes in inflammasome transcriptional activation of speck-like protein containing a caspase recruitment domain (CARD) (ASC, P > 0.05) and caspase-1 (CASP1, P > 0.05). In addition, ATP was not able to modify caspase-1 activity in Ngo-infected MDM but was able to increase pyroptosis (P > 0.01). Notably ATP treatment defined an increase of positive staining for IL-1 beta with a distinctive intracellular pattern of distribution. Collectively, these data demonstrate that ATP induces IL-1 beta secretion by a mechanism not related to the NLRP3/ASC/caspase-1 axis and likely is acting at the level of vesicle trafficking or pore formation.es
dc.description.urihttps://www.hindawi.com/journals/mi/2016/1258504/cta/
dc.identifier.citationMediators of Inflammation, vol. 2016, Article ID 1258504es
dc.identifier.issn0962-9351
dc.identifier.otherhttp://dx.doi.org/10.1155/2016/1258504
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/3346
dc.language.isoenes
dc.publisherHINDAWI PUBLISHINGes
dc.subjectKAPPA-B ACTIVATIONes
dc.subjectPORPHYROMONAS-GINGIVALISes
dc.subjectNLRP3 INFLAMMASOMEes
dc.subjectINTRACELLULAR BACTERIAes
dc.subjectEXTRACELLULAR ATPes
dc.subjectEPITHELIAL-CELLSes
dc.subjectHUMAN PHAGOCYTESes
dc.subjectP2X(7) RECEPTORes
dc.subjectDEATHes
dc.subjectEXPRESSIONes
dc.titleATP Induces IL-1𝛽 Secretion in Neisseria gonorrhoeae-Infected Human Macrophages by a Mechanism Not Related to the NLRP3/ASC/Caspase-1 Axises
dc.typeArtículoes
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