The Transcription Factor ArcA Modulates Salmonella’s Metabolism in Response to Neutrophil Hypochlorous Acid-Mediated Stress

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dc.contributor.authorPardo-Esté, Coral
dc.contributor.authorCastro-Severyn, Juan
dc.contributor.authorI. Krüger, Gabriel
dc.contributor.authorCabezas, Carolina Elizabeth
dc.contributor.authorBriones, Alan Cristóbal
dc.contributor.authorAguirre, Camila
dc.contributor.authorMorales, Naiyulin
dc.contributor.authorBaquedano, Maria Soledad
dc.contributor.authorSulbaran, Yoelvis Noe
dc.contributor.authorHidalgo, Alejandro A.
dc.contributor.authorMeneses, Claudio
dc.contributor.authorPoblete-Castro, Ignacio
dc.contributor.authorCastro-Nallar, Eduardo
dc.contributor.authorValvano, Miguel A.
dc.contributor.authorSaavedra, Claudia P.
dc.date.accessioned2021-09-08T13:57:21Z
dc.date.available2021-09-08T13:57:21Z
dc.date.issued2019-12
dc.descriptionIndexación: Scopuses
dc.description.abstractSalmonella Typhimurium, a bacterial pathogen with high metabolic plasticity, can adapt to different environmental conditions; these traits enhance its virulence by enabling bacterial survival. Neutrophils play important roles in the innate immune response, including the production of microbicidal reactive oxygen species (ROS). In addition, the myeloperoxidase in neutrophils catalyzes the formation of hypochlorous acid (HOCl), a highly toxic molecule that reacts with essential biomolecules, causing oxidative damage including lipid peroxidation and protein carbonylation. The bacterial response regulator ArcA regulates adaptive responses to oxygen levels and influences the survival of Salmonella inside phagocytic cells. Here, we demonstrate by whole transcriptomic analyses that ArcA regulates genes related to various metabolic pathways, enabling bacterial survival during HOCl-stress in vitro. Also, inside neutrophils, ArcA controls the transcription of several metabolic pathways by downregulating the expression of genes related to fatty acid degradation, lysine degradation, and arginine, proline, pyruvate, and propanoate metabolism. ArcA also upregulates genes encoding components of the oxidative pathway. These results underscore the importance of ArcA in ATP generation inside the neutrophil phagosome and its participation in bacterial metabolic adaptations during HOCl stress. © Copyright © 2019 Pardo-Esté, Castro-Severyn, Krüger, Cabezas, Briones, Aguirre, Morales, Baquedano, Sulbaran, Hidalgo, Meneses, Poblete-Castro, Castro-Nallar, Valvano and Saavedra.es
dc.description.urihttps://www.frontiersin.org/articles/10.3389/fmicb.2019.02754/full
dc.identifier.citationFrontiers in Microbiology Volume 105 December 2019 Article number 2754es
dc.identifier.doi10.3389/fmicb.2019.02754
dc.identifier.issn1664-302X
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/20168
dc.language.isoenes
dc.publisherFrontiers Media S.A.es
dc.rights.licenseAtribución 4.0 Internacional (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectArcAes
dc.subjecthypochlorous acides
dc.subjectneutrophilses
dc.subjectSalmonellaes
dc.subjecttranscription factores
dc.titleThe Transcription Factor ArcA Modulates Salmonella’s Metabolism in Response to Neutrophil Hypochlorous Acid-Mediated Stresses
dc.typeArtículoes
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