The complex of PAMAM-OH dendrimer with Angiotensin (1–7) prevented the disuse-induced skeletal muscle atrophy in mice

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dc.contributor.authorMárquez-Miranda, V.
dc.contributor.authorAbrigo, J.
dc.contributor.authorRivera, J.C.
dc.contributor.authorAraya-Durán, I.
dc.contributor.authorAravena, J.
dc.contributor.authorSimon, F.
dc.contributor.authorPacheco, N.
dc.contributor.authorGonzález-Nilo, F.D.
dc.contributor.authorCabello-Verrugio, C.
dc.date.accessioned2017-09-14T20:51:50Z
dc.date.available2017-09-14T20:51:50Z
dc.date.issued2017-03
dc.descriptionIndexación: Web of Science; Scopus.es_CL
dc.description.abstractAngiotensin (1–7) (Ang-(1–7)) is a bioactive heptapeptide with a short half-life and has beneficial effects in several tissues – among them, skeletal muscle – by preventing muscle atrophy. Dendrimers are promising vehicles for the protection and transport of numerous bioactive molecules. This work explored the use of a neutral, non-cytotoxic hydroxyl-terminated poly(amidoamine) (PAMAM-OH) dendrimer as an Ang-(1–7) carrier. Bioinformatics analysis showed that the Ang-(1–7)-binding capacity of the dendrimer presented a 2:1 molar ratio. Molecular dynamics simulation analysis revealed the capacity of neutral PAMAM-OH to protect Ang-(1–7) and form stable complexes. The peptide coverage ability of the dendrimer was between ~50% and 65%. Furthermore, an electrophoretic mobility shift assay demonstrated that neutral PAMAM-OH effectively bonded peptides. Experimental results showed that the Ang-(1–7)/PAMAM-OH complex, but not Ang-(1–7) alone, had an anti-atrophic effect when administered intraperitoneally, as evaluated by muscle strength, fiber diameter, myofibrillar protein levels, and atrogin-1 and MuRF-1 expressions. The results of the Ang-(1–7)/PAMAM-OH complex being intraperitoneally injected were similar to the results obtained when Ang-(1–7) was systemically administered through mini-osmotic pumps. Together, the results suggest that Ang-(1–7) can be protected for PAMAM-OH when this complex is intraperitoneally injected. Therefore, the Ang-(1–7)/PAMAM-OH complex is an efficient delivery method for Ang-(1–7), since it improves the anti-atrophic activity of this peptide in skeletal muscle.es_CL
dc.description.urihttps://www.dovepress.com/the-complex-of-pamam-oh-dendrimer-with-angiotensin-1ndash7-prevented-t-peer-reviewed-article-IJN
dc.identifier.citationInternational Journal of Nanomedicine. Volume 12, 13 March 2017, Pages 1985-1999es_CL
dc.identifier.issn1176-9114
dc.identifier.issn1178-2013
dc.identifier.otherhttps://doi.org/10.2147/IJN.S125521
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/4248
dc.language.isoenes_CL
dc.publisherDove Medical Press Ltd.es_CL
dc.subjectPeptide deliveryes_CL
dc.subjectMuscle wastinges_CL
dc.subjectCarrieres_CL
dc.subjectAnti-atrophic peptidees_CL
dc.titleThe complex of PAMAM-OH dendrimer with Angiotensin (1–7) prevented the disuse-induced skeletal muscle atrophy in micees_CL
dc.typeArtículoes_CL
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