The absence of interleukin 10 affects the morphology, differentiation, granule content and the production of cryptidin-4 in Paneth cells in mice

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dc.contributor.authorBerkowitz, Loni
dc.contributor.authorPardo-Roa, Catalina
dc.contributor.authorRamírez, Gigliola
dc.contributor.authorVallejos, Omar P.
dc.contributor.authorSebastián, Valentina P.
dc.contributor.authorRiedel, Claudia A.
dc.contributor.authorÁlvarez-Lobos, Manuel
dc.contributor.authorBueno, Susan M.
dc.date.accessioned2024-04-15T17:10:02Z
dc.date.available2024-04-15T17:10:02Z
dc.date.issued2019-09-01
dc.descriptionINDEXACIÓN: SCOPUS.
dc.description.abstractPaneth cells (PCs) are specialized epithelial cells of the small bowel that contain multiple secretory granules filled with antimicrobial peptides and trophic factors, which are essential for the control of the microorganisms growth and maintaining intestinal integrity. Alterations in their function are associated with an imbalance of the normal microbiota, gastrointestinal infections and inflammatory processes, such as Crohn's disease (CD). One of the most common murine models for studying CD is IL-10-/- mouse. IL-10-/- mice when housed in conventional conditions and take contact with commensal microorganisms develop an acute enterocolitis mediated by a Th1 immune response. Even though, alterations in PCs function are related to CD, they had not been characterized yet in this mouse model. Here we show that in specific pathogen free conditions IL-10-/- mice have aberrant granules and a large number of immature PCs at the bottom of the crypt in the ileum of IL-10-/- mice before developing intestinal inflammation, along with a reduced expression of Indian Hedgehog. In addition, IL- 10-/- Paneth cells presented a reduced expression of cryptidin-4, and a heterogeneous distribution of lysozyme+ granules. The alterations in the maturation of the PCs at the bottom of the crypt were not modified after the colonization by the conventional microbiota. On the other hand, depletion of microbiota altered the phenotype, but did not normalize PCs. Our results suggest that IL-10 could be necessary for the integrity of PCs. Moreover, our results help to explain why IL-10-/- mice develop enterocolitis in response to microorganisms. © 2019 Berkowitz et al.
dc.identifier.citationPLoS ONE, Volume 14, Issue 9, 1 September 2019, Article number e0221618
dc.identifier.doi10.1371/journal.pone.0221618
dc.identifier.issn1932-6203
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/55982
dc.language.isoen
dc.publisherPublic Library of Science
dc.rights.licenseCC BY 4.0 DEED Atribución 4.0 Internacional
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectcryptidin 4
dc.subjectinterleukin 10
dc.subjectpeptide
dc.subjectsonic hedgehog protein
dc.subjectunclassified drug
dc.titleThe absence of interleukin 10 affects the morphology, differentiation, granule content and the production of cryptidin-4 in Paneth cells in mice
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