Generation and analysis of expressed sequence tags from Botrytis cinerea
| dc.contributor.author | Silva, Evelyn | |
| dc.contributor.author | Valdez, Jorge | |
| dc.contributor.author | Holmes, David | |
| dc.contributor.author | Shmaryahu, Amir | |
| dc.contributor.author | Valenzuela, Pablo | |
| dc.date.accessioned | 2014-06-03T15:06:50Z | |
| dc.date.accessioned | 2016-05-24T18:42:29Z | |
| dc.date.available | 2014-06-03T15:06:50Z | |
| dc.date.available | 2016-05-24T18:42:29Z | |
| dc.date.issued | 2006 | |
| dc.description | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200018&lng=es&nrm=iso | es |
| dc.description.abstract | Botrytis cinerea is a filamentous plant pathogen of a wide range of plant species, and its infection may cause enormous damage both during plant growth and in the post-harvest phase. We have constructed a cDNA library from an isolate of B. cinerea and have sequenced 11,482 expressed sequence tags that were assembled into 1,003 contigs sequences and 3,032 singletons. Approximately 81% of the unigenes showed significant similarity to genes coding for proteins with known functions: more than 50% of the sequences code for genes involved in cellular metabolism, 12% for transport of metabolites, and approximately 10% for cellular organization. Other functional categories include responses to biotic and abiotic stimuli, cell communication, cell homeostasis, and cell development. We carried out pair-wise comparisons with fungal databases to determine the B. cinerea unisequence set with relevant similarity to genes in other fungal pathogenic counterparts. Among the 4,035 non-redundant B. cinerea unigenes, 1,338 (23%) have significant homology with Fusarium verticillioides unigenes. Similar values were obtained for Saccharomyces cerevisiae and Aspergillus nidulans (22% and 24%, respectively). The lower percentages of homology were with Magnaporthe grisae and Neurospora crassa (13% and 19%, respectively). Several genes involved in putative and known fungal virulence and general pathogenicity were identified. The results provide important information for future research on this fungal pathogen. | es |
| dc.identifier.citation | Biol. Res. v.39 n.2 Santiago 2006 | es |
| dc.identifier.issn | 0716-9760 | |
| dc.identifier.other | http://dx.doi.org/10.4067/S0716-97602006000200018 | |
| dc.identifier.uri | http://repositorio.unab.cl/xmlui/handle/ria/2467 | |
| dc.language.iso | en | es |
| dc.publisher | Sociedad de Biología de Chile | es |
| dc.subject | Botrytis cinerea | es |
| dc.subject | cDNA sequencing | es |
| dc.subject | fungal pathogenicity | es |
| dc.title | Generation and analysis of expressed sequence tags from Botrytis cinerea | es |
| dc.type | Artículo | es |
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