Novel Salmonella Phage, vB_Sen_STGO-35-1, Characterization and Evaluation in Chicken Meat

Vista sencilla de ítem
dc.contributor.authorRivera, Dácil
dc.contributor.authorMoreno Switt, Andrea I.
dc.contributor.authorDenes, Thomas G.
dc.contributor.authorHudson, Lauren K.
dc.contributor.authorPeters, Tracey L.
dc.contributor.authorSamir, Reham
dc.contributor.authorAziz, Ramy K.
dc.contributor.authorNoben, Jean-Paul
dc.contributor.authorWagemans, Jeroen
dc.contributor.authorDueñas, Fernando
dc.date.accessioned2022-06-01T21:50:04Z
dc.date.available2022-06-01T21:50:04Z
dc.date.issued2022-03
dc.descriptionIndexación:Scopuses
dc.description.abstractSalmonellosis is one of the most frequently reported zoonotic foodborne diseases world-wide, and poultry is the most important reservoir of Salmonella enterica serovar Enteritidis. The use of lytic bacteriophages (phages) to reduce foodborne pathogens has emerged as a promising bio-control intervention for Salmonella spp. Here, we describe and evaluate the newly isolated Salmonella phage STGO-35-1, including: (i) genomic and phenotypic characterization, (ii) an analysis of the reduction of Salmonella in chicken meat, and (iii) genome plasticity testing. Phage STGO-35-1 repre-sents an unclassified siphovirus, with a length of 47,483 bp, a G + C content of 46.5%, a headful strategy of packaging, and a virulent lifestyle. Phage STGO-35-1 reduced S. Enteritidis counts in chicken meat by 2.5 orders of magnitude at 4 °C. We identified two receptor-binding proteins with affinity to LPS, and their encoding genes showed plasticity during an exposure assay. Phenotypic, proteomic, and genomic characteristics of STGO-35-1, as well as the Salmonella reduction in chicken meat, support the potential use of STGO-35-1 as a targeted biocontrol agent against S. Enteritidis in chicken meat. Additionally, computational analysis and a short exposure time assay allowed us to predict the plasticity of genes encoding putative receptor-binding proteins. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.es
dc.description.urihttps://www.mdpi.com/2076-2607/10/3/606
dc.identifier.citationMicroorganisms Volume 10, Issue 3March 2022 Article number 606es
dc.identifier.doi10.3390/microorganisms10030606
dc.identifier.issn2076-2607
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/22690
dc.language.isoenes
dc.publisherMDPIes
dc.rights.licenseAtribución 4.0 Internacional (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectReceptor-binding proteinses
dc.subjectSalmonella Enteritidises
dc.subjectSalmonella–phage in foodes
dc.subjectSiphoviral morphotypees
dc.subjectSiphoviridaees
dc.titleNovel Salmonella Phage, vB_Sen_STGO-35-1, Characterization and Evaluation in Chicken Meates
dc.typeArtículoes
Archivos
Bloque original
Mostrando 1 - 1 de 1
Miniatura
Nombre:
Rivera_D_Novel_Salmonella_Phage_2022_Article.pdf
Tamaño:
2.44 MB
Formato:
Adobe Portable Document Format
Descripción:
TEXTO COMPLETO EN INGLES
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar
Colecciones
Fac.CV - Artículos de Revista