Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi

Vista sencilla de ítem
dc.contributor.authorFisher, M.C.
dc.contributor.authorGhosh, P.
dc.contributor.authorShelton, J.M.G.
dc.contributor.authorBates, K.
dc.contributor.authorBrookes, L.
dc.contributor.authorWierzbicki, C.
dc.contributor.authorRosa, G.M.
dc.contributor.authorFarrer, R.A.
dc.contributor.authorAanensen, D.M.
dc.contributor.authorAlvarado-Rybak, M.
dc.contributor.authorBataille, A.
dc.contributor.authorBerger, L.
dc.contributor.authorBöll, S.
dc.contributor.authorBosch, J.
dc.contributor.authorClare, F.C.
dc.contributor.authorCourtois, E.A.
dc.contributor.authorCrottini, A.
dc.contributor.authorCunningham, A.A.
dc.contributor.authorDoherty-Bone, T.M.
dc.contributor.authorGebresenbet, F.
dc.contributor.authorGower, D.J.
dc.contributor.authorHöglund, J.
dc.contributor.authorJames, T.Y.
dc.contributor.authorJenkinson, T.S.
dc.contributor.authorKosch, T.A.
dc.contributor.authorLambertini, C.
dc.contributor.authorLaurila, A.
dc.contributor.authorLin, C.-F.
dc.contributor.authorLoyau, A.
dc.contributor.authorMartel, A.
dc.contributor.authorMeurling, S.
dc.contributor.authorMiaud, C.
dc.contributor.authorMinting, P.
dc.contributor.authorNdriantsoa, S.
dc.contributor.authorO'Hanlon, S.J.
dc.contributor.authorPasmans, F.
dc.contributor.authorRakotonanahary, T.
dc.contributor.authorRabemananjara, F.C.E.
dc.contributor.authorRibeiro, L.P.
dc.contributor.authorSchmeller, D.S.
dc.contributor.authorSchmidt, B.R.
dc.contributor.authorSkerratt, L.
dc.contributor.authorSmith, F.
dc.contributor.authorSoto-Azat, C.
dc.contributor.authorTessa, G.
dc.contributor.authorToledo, L.F.
dc.contributor.authorValenzuela-Sánchez, A.
dc.contributor.authorVerster, R.
dc.contributor.authorVörös, J.
dc.contributor.authorWaldman, B.
dc.contributor.authorWebb, R.J.
dc.contributor.authorWeldon, C.
dc.contributor.authorWombwell, E.
dc.contributor.authorZamudio, K.R.
dc.contributor.authorLongcore, J.E.
dc.contributor.authorGarner, T.W.J.
dc.date.accessioned2019-05-28T16:05:26Z
dc.date.available2019-05-28T16:05:26Z
dc.date.issued2018-12
dc.descriptionIndexación: Scopus.es_ES
dc.description.abstractParasitic chytrid fungi have emerged as a significant threat to amphibian species worldwide, necessitating the development of techniques to isolate these pathogens into culture for research purposes. However, early methods of isolating chytrids from their hosts relied on killing amphibians. We modified a pre-existing protocol for isolating chytrids from infected animals to use toe clips and biopsies from toe webbing rather than euthanizing hosts, and distributed the protocol to researchers as part of the BiodivERsA project RACE; here called the RML protocol. In tandem, we developed a lethal procedure for isolating chytrids from tadpole mouthparts. Reviewing a database of use a decade after their inception, we find that these methods have been applied across 5 continents, 23 countries and in 62 amphibian species. Isolation of chytrids by the non-lethal RML protocol occured in 18% of attempts with 207 fungal isolates and three species of chytrid being recovered. Isolation of chytrids from tadpoles occured in 43% of attempts with 334 fungal isolates of one species (Batrachochytrium dendrobatidis) being recovered. Together, these methods have resulted in a significant reduction and refinement of our use of threatened amphibian species and have improved our ability to work with this group of emerging pathogens. © 2018 The Author(s).es_ES
dc.description.urihttps://www.nature.com/articles/s41598-018-24472-2
dc.identifier.citationScientific Reports, 8(1), art. no. 7772es_ES
dc.identifier.issn2045-2322
dc.identifier.otherDOI: 10.1038/s41598-018-24472-2
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/8921
dc.language.isoenes_ES
dc.publisherNature Publishing Groupes_ES
dc.subjectAnimal experimentes_ES
dc.subjectAnimal tissuees_ES
dc.subjectBatrachochytrium dendrobatidises_ES
dc.subjectInfectious agentes_ES
dc.subjectNonhumanes_ES
dc.subjectTadpolees_ES
dc.titleDevelopment and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungies_ES
dc.typeArtículoes_ES
Archivos
Bloque original
Mostrando 1 - 1 de 1
Miniatura
Nombre:
Fisher_Development_and_worldwide.pdf
Tamaño:
1.29 MB
Formato:
Adobe Portable Document Format
Descripción:
TEXTO COMPLETO EN INGLES
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar
Colecciones
Fac.CV - Artículos de Revista