Examinando por Autor "Maldonado, Paula"
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Ítem Alloreactive regulatory T cells allow the generation of mixed chimerism and transplant tolerance(Frontiers Research Foundation, 2015-11) Ruiz, Paulina; Maldonado, Paula; Hidalgo, Yessia; Sauma, Daniela; Rosemblatt, Mario; Bono, Maria RosaThe induction of donor-specific transplant tolerance is one of the main goals of modern immunology. Establishment of a mixed chimerism state in the transplant recipient has proven to be a suitable strategy for the induction of long-term allograft tolerance; however, current experimental recipient preconditioning protocols have many side effects, and are not feasible for use in future therapies. In order to improve the current mixed chimerism induction protocols, we developed a non-myeloablative bone-marrow transplant (NM-BMT) protocol using retinoic acid (RA)-induced alloantigen-specific Tregs, clinically available immunosuppressive drugs, and lower doses of irradiation. We demonstrate that RA-induced alloantigen-specific Tregs in addition to a NM-BMT protocol generates stable mixed chimerism and induces tolerance to allogeneic secondary skin allografts in mice. Therefore, the establishment of mixed chimerism through the use of donor-specific Tregs rather than non-specific immunosuppression could have a potential use in organ transplantation. © 2015 Ruiz, Maldonado, Hidalgo, Sauma, Rosemblatt and Bono.Ítem Alloreactive regulatory T cells generated with retinoic acid prevent skin allograft rejection(Wiley-VCH Verlag, 2015-02) Moore, Carolina; Tejon, Gabriela; Fuentes, Camila; Hidalgo, Yessia; Bono, Maria R.; Maldonado, Paula; Fernandez, Ricardo; Wood, Kathryn J.; Fierro, Juan A.; Rosemblatt, Mario; Sauma, Daniela; Bushell, AndrewCD4+CD25+Foxp3+ regulatory T (Treg) cells mediate immunological self-tolerance and suppress immune responses. Retinoic acid (RA), a natural metabolite of vitamin A, has been reported to enhance the differentiation of Treg cells in the presence of TGF-β. In this study, we show that the co-culture of naive T cells from C57BL/6 mice with allogeneic antigen-presenting cells (APCs) from BALB/c mice in the presence of TGF-β, RA, and IL-2 resulted in a striking enrichment of Foxp3+ T cells. These RA in vitro-induced regulatory T (RA-iTreg) cells did not secrete Th1-, Th2-, or Th17-related cytokines, showed a nonbiased homing potential, and expressed several cell surface molecules related to Treg-cell suppressive potential. Accordingly, these RA-iTreg cells suppressed T-cell proliferation and inhibited cytokine production by T cells in in vitro assays. Moreover, following adoptive transfer, RA-iTreg cells maintained Foxp3 expression and their suppressive capacity. Finally, RA-iTreg cells showed alloantigen-specific immunosuppressive capacity in a skin allograft model in immunodeficient mice. Altogether, these data indicate that functional and stable allogeneic-specific Treg cells may be generated using TGF-β, RA, and IL-2. Thus, RA-iTreg cells may have a potential use in the development of more effective cellular therapies in clinical transplantation. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.Ítem Aprendizaje emocional en las escuelas(Universidad Andrés Bello, 2002) Almendra, Natalia; Andrada, Carmen; Cambiaso, Grace; Cubillos, Josué; Matus, Jeannette; Maldonado, Paula; Polo, Erica; Sepúlveda., Carmen; Aravena Ricardi, Nancy; Facultad de EducaciónEl objeto de nuestro trabajo es analizar el concepto de inteligencia emocional dentro del proceso de enseñanza y aprendizaje para mejorar el rendimiento académico en niños y niñas de Educación General Básica. Esta investigación proyectará a partir del objetivo general cuatro objetivos específicos los cuales nos permitirían a través de este diseño llevar a abordar esta problemática y su incidencia en el aprendizaje escolar.