Examinando por Autor "Mellet, Carmen Ortiz"

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    Molecular determinants for cyclo-oligosaccharide-based nanoparticle-mediated effective siRNA transfection
    (Future Medicine Ltd., 2017-07) Manzanares, Darío; Araya-Durán, Ingrid; Gallego-Yerga, Laura; Játiva, Pablo; Márquez-Miranda, Valeria; Canan, Jonathan; Jiménez Blanco, José Luis; Mellet, Carmen Ortiz; González-Nilo, Fernando Danilo; García Fernández, José Manuel; Ceña, Valentín
    Aim: To study the structural requirements that a cyclooligosaccharide-based nanoparticle must fulfill to be an efficient siRNA transfection vector. Materials & methods: siRNA protection from degradation by RNAses, transfection efficiency and the thermodynamic parameters of the nanoparticle/siRNA interactions were studied on pairs of amphiphilic molecules using biochemical techniques and molecular dynamics. Results: The lower the siRNA solvent accessible surface area in the presence of the nanoparticle, higher the protection from RNAse-mediated degradation in the corresponding nanocomplex; a moderate nanoparticle/siRNA binding energy value further facilitates reversible complexation and binding to the target cellular mRNA. Conclusion: The use, in advance, of these parameters will provide a useful indication of the potential of a molecular nanoparticle as siRNA transfecting vector. © 2017 Future Medicine Ltd.