Examinando por Autor "Moreno-Switt, Andrea I."
Mostrando 1 - 6 de 6
Resultados por página
Opciones de ordenación
Ítem Comparative analysis of Felixounavirus genomes including two new members of the genus that infect Salmonella infantis(MDPI, 2021-07) Barron-Montenegro, Rocío; García, Rodrigo; Dueñas, Fernando; Rivera, Dácil; Opazo-Capurro, Andrés; Erickson, Stephen; Moreno-Switt, Andrea I.Salmonella spp. is one of the most common foodborne pathogens worldwide; therefore, its control is highly relevant for the food industry. Phages of the Felixounavirus genus have the characteristic that one phage can infect a large number of different Salmonella serovars and, thus, are proposed as an alternative to antimicrobials in food production. Here, we describe two new members of the Felixounavirus genus named vB_Si_35FD and vB_Si_DR94, which can infect Salmonella Infantis. These new members were isolated and sequenced, and a subsequent comparative genomic analysis was conducted including 23 publicly available genomes of Felixounaviruses that infect Salmonella. The genomes of vB_Si_35FD and vB_Si_DR94 are 85,818 and 85,730 bp large and contain 129 and 125 coding sequences, respectively. The genomes did not show genes associated with virulence or antimicrobial resistance, which could be useful for candidates to use as biocontrol agents. Comparative genomics revealed that closely related Felixounavirus are found in distinct geographical locations and that this genus has a conserved genomic structure despite its worldwide distribution. Our study revealed a highly conserved structure of the phage genomes, and the two newly described phages could represent promising biocontrol candidates against Salmonella spp. from a genomic viewpoint. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.Ítem ECOPHAGE: Combating Antimicrobial Resistance Using Bacteriophages for Eco-Sustainable Agriculture and Food Systems(Multidisciplinary Digital Publishing Institute (MDPI), 2023-11) García, Pilar; Tabla, Rafael; Anany, Hany; Bastias, Roberto; Brøndsted, Lone; Casado, Susana; Cifuentes, Pablo; Deaton, John; Denes, Thomas G.; Islam, Mohammad Aminul; Lavigne, Rob; Moreno-Switt, Andrea I.; Nakayama, Natsuko; Muñoz Madero, Cristina; Sulakvelidze, Alexander; Svircev, Antonet M.; Wagemans, Jeroen; Biosca, Elena G.; Rivera, DácilThe focus of this meeting was to discuss the suitability of using bacteriophages as alternative antimicrobials in the agrifood sector. Following a One Health approach, the workshop explored the possibilities of implementing phage application strategies in the agriculture, animal husbandry, aquaculture, and food production sectors. Therefore, the meeting had gathered phage researchers, representatives of the agrifood industry, and policymakers to debate the advantages and potential shortcomings of using bacteriophages as alternatives to traditional antimicrobials and chemical pesticides. Industry delegates showed the latest objectives and demands from consumers. Representatives of regulatory agencies (European Medicines Agency (EMA) and Spanish Agency of Medicines and Health Products (AEMPS)) presented an update of new regulatory aspects that will impact and support the approval and implementation of phage application strategies across the different sectors.Ítem Higher prevalence of extended-spectrum cephalosporin-resistant enterobacterales in dogs attended for enteric viruses in brazil before and after treatment with cephalosporins(MDPI AG, 2021-02) Salgado-Caxito, Marília; Moreno-Switt, Andrea I.; Paes, Antonio Carlos; Shiva, Carlos; Munita, Jose M.; Rivas, Lina; Benavides, Julio A.The extensive use of antibiotics is a leading cause for the emergence and spread of antimicrobial resistance (AMR) among dogs. However, the impact of using antibiotics to treat viral infections on AMR remains unknown. In this study, we compared the prevalence of extended-spectrum cephalosporin-resistant Enterobacterales (ESCR-E) between dogs with a suspected infection of canine parvovirus (CPV) and canine distemper (CDV) before and after treatment with third-generation cephalosporins. We found a higher prevalence of ESCR-E faecal carriage in dogs suspected of CPV (37%) and CDV (15%) compared to dogs with noninfectious pathologies (9%) even prior to the start of their treatment. A 7-day course of ceftriaxone or ceftiofur administrated to CPV and CDV-suspected dogs substantially increased their ESCR-E faecal carriage during treatment (85% for CPV and 57% for CDV), and 4 weeks after the treatment ended (89% for CPV and 60% for CDV) when dogs were back in their households. Most of the observed resistance was carried by ESCR-E. coli carrying blaCTX-M genes. Our results suggest the need to optimize prophylactic antibiotic therapy in dogs treated for a suspected viral infection to prevent ESCR-E emergence and spread in the community.Ítem Isolation of Ciprofloxacin and Ceftazidime-Resistant Enterobacterales From Vegetables and River Water Is Strongly Associated With the Season and the Sample Type(Frontiers Media S.A, 2021-09) Díaz Gavidia, Constanza; Barría, Carla; Rivas, Lina; García, Patricia; Alvarez, Francisca P.; González Rocha, Gerardo; Opazo Capurro, Andrés; Araos, Rafael; Munita, José M.; Cortes, Sandra; Olivares Pacheco, Jorge; Adell, Aiko D.; Moreno-Switt, Andrea I.The dissemination of antibiotic-resistant bacteria (ARB) from water used for crop irrigation to vegetables is poorly studied. During a year, five farmer markets in a city in Central Chile were visited, and 478 vegetable samples (parsleys, corianders, celeries, lettuces, chards, and beets) were collected. Simultaneously, 32 water samples were collected from two rivers which are used to irrigate the vegetables produced in the area. Resistant Enterobacterales were isolated and identified. Colistin resistance gene mcr-1 and extended spectrum β-lactamases (ESBL) were molecularly detected. The association of environmental factors was evaluated, with the outcomes being the presence of Enterobacterales resistant to four antibiotic families and the presence of multidrug resistance (MDR) phenotypes. Parsley, coriander, and celery showed the highest prevalence of resistant Enterobacterales (41.9% for ciprofloxacin and 18.5% for ceftazidime). A total of 155 isolates were obtained, including Escherichia coli (n=109), Citrobacter sp. (n=20), Enterobacter cloacae complex (n=8), Klebsiella pneumoniae (n=8), and Klebsiella aerogenes (n=1). Resistance to ampicillin (63.2%) and ciprofloxacin (74.2%) was most frequently found; 34.5% of the isolates showed resistance to third-generation cephalosporins, and the MDR phenotype represented 51.6% of the isolates. In two E. coli isolates (1.29%), the gene mcr-1 was found and ESBL genes were found in 23/62 isolates (37%), with blaCTX-M being the most frequently found in 20 isolates (32%). Resistant Enterobacterales isolated during the rainy season were less likely to be MDR as compared to the dry season. Understanding environmental associations represent the first step toward an improved understanding of the public health impact of ARB in vegetables and water. © Copyright © 2021 Díaz-Gavidia, Barría, Rivas, García, Alvarez, González-Rocha, Opazo-Capurro, Araos, Munita, Cortes, Olivares-Pacheco, Adell and Moreno-Switt.Ítem Perspective on Clinically-Relevant Antimicrobial Resistant Enterobacterales in Food: Closing the Gaps Using Genomics(Frontiers Media S.A., 2021-05) Díaz-Gavidia, Constanza; Álvarez, Francisca P.; Munita, Jose M.; Cortés, Sandra; Moreno-Switt, Andrea I.Antimicrobial resistance is one of the most important public health concerns—it causes 700,000 deaths annually according to the World Health Organization (WHO). Enterobacterales such as E. coli and Klebsiella pneumoniae, have become resistant to many relevant antimicrobials including carbapenems and extended spectrum cephalosporins. These clinically relevant resistant Enterobacterales (CRRE) members are now globally distributed in the environment including different food types (meats, produce, dairy). Unlike known foodborne pathogens, CRRE are not usually part of most food surveillance systems. However, numerous reports of CRRE highlight the importance of these bacteria in food and have been shown to contribute to the overall crisis of antimicrobial resistance. This is especially important in the context of carriage of these pathogens by immuno-compromised individuals. CRRE infections upon consumption of contaminated food could colonize the human gastrointestinal tract and eventually be a source of systemic infections such as urinary tract infections or septicemia. While different aspects need to be considered to elucidate this, whole genome sequencing along with metadata could be used to understand genomic relationships of CRRE obtained from foods and humans, including isolates from clinical infections. Once robust scientific data is available on the role of CRRE in food, countries could move forward to better survey and control CRRE in food. © Copyright © 2021 Díaz-Gavidia, Álvarez, Munita, Cortés and Moreno-Switt.Ítem Transduction as a Potential Dissemination Mechanism of a Clonal qnrB19-Carrying Plasmid Isolated From Salmonella of Multiple Serotypes and Isolation Sources (Frontiers in Microbiology, (2019), 10, 10.3389/fmicb.2019.02503)(Frontiers Media S.AFrontiers in MicrobiologyOpen AccessVolume 117 April 2020 Article number 547, 2020-04) Moreno-Switt, Andrea I.; Pezoa, David; Sepúlveda, Vanessa; González, Iván; Rivera, Dácil; Retamal, Patricio; Navarrete, Paola; Reyes-Jara, Angélica; Toro, MagalyIn the original article, “Andres et al. (2013)” was not cited and referenced in the article. The citation has now been inserted in the Introduction, paragraph three and in the Discussion section, paragraph four and should read. © Copyright © 2020 Moreno-Switt, Pezoa, Sepúlveda, González, Rivera, Retamal, Navarrete, Reyes-Jara and Toro.