Examinando por Autor "Olguín, Yusser"

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    Effect of Electrical Stimulation on PC12 Cells Cultured in Different Hydrogels: Basis for the Development of Biomaterials in Peripheral Nerve Tissue Engineering
    (MDPI, 2023-12) Olguín, Yusser; Selva, Mónica; Benavente, Diego; Orellana, Nicole; Montenegro, Ivan; Madrid, Alejandro; Jaramillo-Pinto, Diego; Otero, María Carolina; Corrales, Tomas P.; Acevedo, Cristian A.
    Extensive damage to peripheral nerves is a health problem with few therapeutic alternatives. In this context, the development of tissue engineering seeks to obtain materials that can help recreate environments conducive to cellular development and functional repair of peripheral nerves. Different hydrogels have been studied and presented as alternatives for future treatments to emulate the morphological characteristics of nerves. Along with this, other research proposes the need to incorporate electrical stimuli into treatments as agents that promote cell growth and differentiation; however, no precedent correlates the simultaneous effects of the types of hydrogel and electrical stimuli. This research evaluates the neural differentiation of PC12 cells, relating the effect of collagen, alginate, GelMA, and PEGDA hydrogels with electrical stimulation modulated in four different ways. Our results show significant correlations for different cultivation conditions. Electrical stimuli significantly increase neural differentiation for specific experimental conditions dependent on electrical frequency, not voltage. These backgrounds allow new material treatment schemes to be formulated through electrical stimulation in peripheral nerve tissue engineering. © 2023 by the authors.
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    Ítem
    Osteoconductive Effect of a Nanocomposite Membrane Treated with UV Radiation
    (MDPI, 2022-01) Olguín, Yusser; Acuna-Mendoza, Soledad; Otero, Carolina; Acevedo, Cristian A.; Covarrubias, Cristian
    Modulation of the bio-regenerative characteristics of materials is an indispensable re-quirement in tissue engineering. Particularly, in bone tissue engineering, the promotion of the osteoconductive phenomenon determines the elemental property of a material be used therapeuti-cally. In addition to the chemical qualities of the constituent materials, the three-dimensional surface structure plays a fundamental role that various methods are expected to modulate in a number of ways, one most promising of which is the use of different types of radiation. In the present manuscript, we demonstrate in a calvarial defect model, that treatment with ultraviolet irradiation allows modification of the osteoconductive characteristics in a biomaterial formed by gelatin and chitosan, together with the inclusion of hydroxyapatite and titanium oxide nanoparticles. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.