Examinando por Autor "Pezoa, David"

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    Identification and distribution of new candidate T6SS effectors encoded in Salmonella Pathogenicity Island 6
    (Frontiers Media SA, 2023) Blondel, Carlos J.; Amaya, Fernando A.; Bustamante, Paloma; Santiviago, Carlos A.; Pezoa, David
    The type VI secretion system (T6SS) is a contact-dependent contractile multiprotein apparatus widely distributed in Gram-negative bacteria. These systems can deliver different effector proteins into target bacterial and/or eukaryotic cells, contributing to the environmental fitness and virulence of many bacterial pathogens. Salmonella harbors five different T6SSs encoded in different genomic islands. The T6SS encoded in Salmonella Pathogenicity Island 6 (SPI-6) contributes to Salmonella competition with the host microbiota and its interaction with infected host cells. Despite its relevance, information regarding the total number of effector proteins encoded within SPI-6 and its distribution among different Salmonella enterica serotypes is limited. In this work, we performed bioinformatic and comparative genomics analyses of the SPI-6 T6SS gene cluster to expand our knowledge regarding the T6SS effector repertoire and the global distribution of these effectors in Salmonella. The analysis of a curated dataset of 60 Salmonella enterica genomes from the Secret6 database revealed the presence of 23 new putative T6SS effector/immunity protein (E/I) modules. These effectors were concentrated in the variable regions 1 to 3 (VR1-3) of the SPI-6 T6SS gene cluster. VR1-2 were enriched in candidate effectors with predicted peptidoglycan hydrolase activity, while VR3 was enriched in candidate effectors of the Rhs family with C-terminal extensions with predicted DNase, RNase, deaminase, or ADP-ribosyltransferase activity. A global analysis of known and candidate effector proteins in Salmonella enterica genomes from the NCBI database revealed that T6SS effector proteins are differentially distributed among Salmonella serotypes. While some effectors are present in over 200 serotypes, others are found in less than a dozen. A hierarchical clustering analysis identified Salmonella serotypes with distinct profiles of T6SS effectors and candidate effectors, highlighting the diversity of T6SS effector repertoires in Salmonella enterica. The existence of different repertoires of effector proteins suggests that different effector protein combinations may have a differential impact on the environmental fitness and pathogenic potential of these strains. Copyright © 2023 Blondel, Amaya, Bustamante, Santiviago and Pezoa.
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    Transduction as a Potential Dissemination Mechanism of a Clonal qnrB19-Carrying Plasmid Isolated From Salmonella of Multiple Serotypes and Isolation Sources (Frontiers in Microbiology, (2019), 10, 10.3389/fmicb.2019.02503)
    (Frontiers Media S.AFrontiers in MicrobiologyOpen AccessVolume 117 April 2020 Article number 547, 2020-04) Moreno-Switt, Andrea I.; Pezoa, David; Sepúlveda, Vanessa; González, Iván; Rivera, Dácil; Retamal, Patricio; Navarrete, Paola; Reyes-Jara, Angélica; Toro, Magaly
    In the original article, “Andres et al. (2013)” was not cited and referenced in the article. The citation has now been inserted in the Introduction, paragraph three and in the Discussion section, paragraph four and should read. © Copyright © 2020 Moreno-Switt, Pezoa, Sepúlveda, González, Rivera, Retamal, Navarrete, Reyes-Jara and Toro.
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    Transfer of T6SSSPI-19from Salmonella Gallinarum to Salmonella Typhimurium Lacking T6SSSPI-6Complements its Colonization Defect in Mice
    (Sciendo, 2023-06) Pezoa, David; Blondel, Carlos J.; Amaya, Fernando A.; Santiviago, Carlos A.
    Salmonella genus harbors five Type VI Secretion System (T6SS) gene clusters. The T6SS encoded in SPI-6 (T6SSSPI-6) contributes to Salmonella Typhimurium colonization of chickens and mice, while the T6SS encoded in SPI-19 (T6SSSPI-19) of Salmonella Gallinarum contributes to chicken colonization. Interestingly, the T6SSSPI-19 of Salmonella Gallinarum complemented the defect in chicken colonization of a Salmonella Typhimurium strain that lacks the T6SSSPI-6, suggesting that both T6SSs are interchangeable. Here we show that the transfer of Salmonella Gallinarum T6SSSPI-19 complemented the defect in mice colonization of a Salmonella Typhimurium ΔT6SSSPI-6 strain, indicating that both T6SSs are functionally redundant during host colonization. © 2023 David Pezoa et al., published by Sciendo.