Examinando por Autor "Sánchez, Mario"
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Ítem Cardiopulmonary- versus neuromuscular-based high-intensity interval training during a pre-season in youth female basketball players(Wroclaw University of Health and Sport Sciences, 2023) Rodríguez-Fernández, Alejandro; Lago, Ángel; Ramirez-Campillo, Rodrigo; Sánchez, Mario; Sánchez-Sánchez, JavierPurpose. This study compares the effects of 6 weeks of pre-season cardiopulmonary- vs. neuromuscular-based high-intensity interval training (HIIT) in female basketball players’ physical fitness. Methods. Overall, 16 female regional-level U19 basketball players were randomly allocated to a cardiopulmonary-based HIIT (C-HIIT) group (n = 8; age: 17.9 ± 0.6 years; height: 175.4 ± 6.5 cm; body mass: 68.1 ± 7.2 kg) or a neuromuscular-based HIIT (N-HIIT) group (n= 8; age: 18.0 ± 0.4 years; height: 175.6 ± 3.6 cm; body mass: 69.8 ± 5.0 kg). At the PRE-, MID- (3 weeks), and POST-training time points, the participants performed a 30-15 Intermittent Fitness Test (30-15 IFT; aerobic performance) and a repeated sprint ability (RSA) test (6 × 15 + 15 m [with 180° change of direction] with 20-second passive recovery). Results. A 2 × 3 mixed model analysis of variance revealed that both training interventions improved (p < 0.05) 30-15 IFT after 6 weeks (C-HIIT, 8.6% and ES = 2.01; N-HIIT, 3.3% and ES = 0.52). C-HIIT improved (p < 0.05) 30-15 IFT after 3 weeks (3.8%, ES = 0.90). Neither group improved (p > 0.05) in RSA results. Conclusions. C-HIIT and N-HIIT improves aerobic performance in female basketball players. However, for improvements in RSA, other training drills (e.g., repeated sprint training) and tasks with a higher number of changes of direction may be investigated. © Wroclaw University of Health and Sport Sciences.Ítem Electroablation: A method for neurectomy and localized tissue injury(BMC, 2014-02) Moya-Díaz, José; Peña, Oscar A.; Sánchez, Mario; Ureta, Daniela A; Reynaert, Nicole G; Anguita-Salinas, Consuelo; Marín, Gonzalo; Allende, Miguel LAbstract Background: Tissue injury has been employed to study diverse biological processes such as regeneration and inflammation. In addition to physical or surgical based methods for tissue injury, current protocols for localized tissue damage include laser and two-photon wounding, which allow a high degree of accuracy, but are expensive and difficult to apply. In contrast, electrical injury is a simple and inexpensive technique, which allows reproducible and localized cell or tissue damage in a variety of contexts. Results: We describe a novel technique that combines the advantages of zebrafish for in vivo visualization of cells with those of electrical injury methods in a simple and versatile protocol which allows the study of regeneration and inflammation. The source of the electrical pulse is a microelectrode that can be placed with precision adjacent to specific cells expressing fluorescent proteins. We demonstrate the use of this technique in zebrafish larvae by damaging different cell types and structures. Neurectomy can be carried out in peripheral nerves or in the spinal cord allowing the study of degeneration and regeneration of nerve fibers. We also apply this method for the ablation of single lateral line mechanosensory neuromasts, showing the utility of this approach as a tool for the study of organ regeneration. In addition, we show that electrical injury induces immune cell recruitment to damaged tissues, allowing in vivo studies of leukocyte dynamics during inflammation within a confined and localized injury. Finally, we show that it is possible to apply electroablation as a method of tissue injury and inflammation induction in adult fish. Conclusions: Electrical injury using a fine microelectrode can be used for axotomy of neurons, as a general tissue ablation tool and as a method to induce a powerful inflammatory response. We demonstrate its utility to studies in both larvae and in adult zebrafish but we expect that this technique can be readily applied to other organisms as well. We have called this method of electrical based tissue ablation, electroablation.Ítem Factors associated to the market value of professional soccer players(Universidad de Murcia Servicio de Publicaciones, 2022) Sánchez, Mario; Orgaz, Begoña; Ramirez-Campillo, Rodrigo; Nakamura, Fabio Yuzo; Luis-Pereira, Jesús María; Carretero, Manuel; Sánchez-Sánchez, JavierThe objective of this study was analysed to the market value (MV) of professional soccer players from Top-5 European Soccer Leagues. An ANOVA analysed the effect of the independent variables on the MV of the soccer players, with p<0.01. Soccer players from the Premier League (PL) showed greater MV compared to other leagues. Foreign soccer players showed greater MV compared to national soccer players. Soccer players between 21-30 years showed greater MV compared to those <21 and >30 years. Forward and midfielders showed greater MV compared to goalkeepers and backs. Soccer players that represented their countries at international matches showed greater MV compared to those that did not. The soccer players with a youth background formation in their clubs in the Premier League and La Liga showed greater MV compared to soccer players coming from other clubs. In conclusion, extrinsic factors affect the MV of soccer players. Research results may be useful to the sport managers in the selection of professional soccer players. © Copyright 2018: Servicio de Publicaciones de la Universidad de MurciaÍtem Identification of LOC101927355 as a Novel Biomarker for Preeclampsia(MDPI, 2022-06) Peñailillo, Reyna; Monteiro, Lara J.; Acuña-Gallardo, Stephanie; García, Felipe; Velásquez, Victoria; Correa, Paula; Díaz, Pilar; Valdebenito, Patricia P.; Navarro, Cristina; Romero, Roberto; Sánchez, Mario; Illanes, Sebastián E.; Nardocci, GinoPreeclampsia, a disorder with a heterogeneous physiopathology, can be attributed to mater-nal, fetal, and/or placental factors. Long non-coding RNAs (lncRNAs) refer to a class of non-coding RNAs, the essential regulators of biological processes; their differential expression has been associated with the pathogenesis of multiple diseases. The study aimed to identify lncRNAs, expressed in the placentas and plasma of patients who presented with preeclampsia, as potential putative biomarkers of the disease. In silico analysis was performed to determine lncRNAs differentially expressed in the placentas of patients with preeclampsia, using a previously published RNA-Seq dataset. Seven placentas and maternal plasma samples collected at delivery from preterm preeclamptic patients (≤37 gestational weeks of gestation), and controls were used to validate the expression of lncRNAs by qRT-PCR. Six lncRNAs were validated and differentially expressed (p < 0.05) in the preeclampsia and control placentas: UCA1 and HCG4 were found upregulated, and LOC101927355, LINC00551, PART1, and NRAD1 downregulated. Two of these lncRNAs, HCG4 and LOC101927355, were also detected in maternal plasma, the latter showing a significant decrease (p = 0.03) in preeclamptic patients compared to the control group. In silico analyses showed the cytoplasmic location of LOC101927355, which suggests a role in post-transcriptional gene regulation. The detection of LOC101927355 in the placenta and plasma opens new possibilities for understanding the pathogenesis of preeclampsia and for its potential use as a biomarker. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.Ítem Kinetic aspects on ferric arsenate formation in a fix bed gas-solid reaction system(Universidad Nacional de Colombia, 2015) Balladares, Eduardo; Parra, Roberto; Sánchez, MarioThe fixation of arsenic contained in gases produced during pyrometallurgical processes by using solid ferric oxide was studied in the range 873-1073 K under different oxygen potential and solid aggregates porosities. Arsenic fixation on solid iron oxides is described by the pore blocking model under the studied conditions. The solid product of the reaction has a molar volume 3 times larger than the solid reactant causing fast decreasing of the inter-granular spacing. The activation energies of arsenic fixation reaction are 34.96 and 35.46 kJ/mol for porosities of 0.88 and 0.74 respectively, and for porosity of 0.55 the activation energy was 26.88 kJ/mol. These values of activation energy show that intra-pellets diffusion has an effect only in samples with 0.55 porosity. Minor sintering of particles was detected. Industrial application of the concept demands a reaction system, which in is required better gas-solid contact for attaining larger conversions. © The author; licensee Universidad Nacional de Colombia.Ítem Transcriptional and Histone Acetylation Changes Associated with CRE Elements Expose Key Factors Governing the Regulatory Circuit in the Early Stage of Huntington’s Disease Models(MDPI, 2023-06) Arancibia-Opazo, Sandra; Contreras-Riquelme, J. Sebastián; Sánchez, Mario; Cisternas-Olmedo, Marisol; Vidal, René L.; Martin, Alberto J. M.; Sáez, Mauricio A.Huntington’s disease (HD) is a disorder caused by an abnormal expansion of trinucleotide CAG repeats within the huntingtin (Htt) gene. Under normal conditions, the CREB Binding Protein interacts with CREB elements and acetylates Lysine 27 of Histone 3 to direct the expression of several genes. However, mutant Htt causes depletion of CBP, which in turn induces altered histone acetylation patterns and transcriptional deregulation. Here, we have studied a differential expression analysis and H3K27ac variation in 4- and 6-week-old R6/2 mice as a model of juvenile HD. The analysis of differential gene expression and acetylation levels were integrated into Gene Regulatory Networks revealing key regulators involved in the altered transcription cascade. Our results show changes in acetylation and gene expression levels that are related to impaired neuronal development, and key regulators clearly defined in 6-week-old mice are proposed to drive the downstream regulatory cascade in HD. Here, we describe the first approach to determine the relationship among epigenetic changes in the early stages of HD. We determined the existence of changes in pre-symptomatic stages of HD as a starting point for early onset indicators of the progression of this disease. © 2023 by the authors.