Electroablation: A method for neurectomy and localized tissue injury
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Fecha
2014-02
Profesor/a Guía
Facultad/escuela
Idioma
en
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ISSN de la revista
Título del volumen
Editor
BMC
Nombre de Curso
Licencia CC
Atribution 4.0 International (CC BY 4.0)
Licencia CC
https://creativecommons.org/licenses/by/4.0/deed.es
Resumen
Abstract
Background: Tissue injury has been employed to study diverse biological processes such as regeneration and
inflammation. In addition to physical or surgical based methods for tissue injury, current protocols for localized
tissue damage include laser and two-photon wounding, which allow a high degree of accuracy, but are expensive
and difficult to apply. In contrast, electrical injury is a simple and inexpensive technique, which allows reproducible
and localized cell or tissue damage in a variety of contexts.
Results: We describe a novel technique that combines the advantages of zebrafish for in vivo visualization of cells
with those of electrical injury methods in a simple and versatile protocol which allows the study of regeneration
and inflammation. The source of the electrical pulse is a microelectrode that can be placed with precision adjacent
to specific cells expressing fluorescent proteins. We demonstrate the use of this technique in zebrafish larvae by
damaging different cell types and structures. Neurectomy can be carried out in peripheral nerves or in the spinal
cord allowing the study of degeneration and regeneration of nerve fibers. We also apply this method for the
ablation of single lateral line mechanosensory neuromasts, showing the utility of this approach as a tool for the
study of organ regeneration. In addition, we show that electrical injury induces immune cell recruitment to
damaged tissues, allowing in vivo studies of leukocyte dynamics during inflammation within a confined and
localized injury. Finally, we show that it is possible to apply electroablation as a method of tissue injury and
inflammation induction in adult fish.
Conclusions: Electrical injury using a fine microelectrode can be used for axotomy of neurons, as a general tissue
ablation tool and as a method to induce a powerful inflammatory response. We demonstrate its utility to studies in
both larvae and in adult zebrafish but we expect that this technique can be readily applied to other organisms as
well. We have called this method of electrical based tissue ablation, electroablation.
Notas
Indexación: Scopus.
Palabras clave
Axotomy, Neurectomy, Tissue Ablation, Regeneration, Inflammation, Zebrafish
Citación
BMC Developmental Biology. Volume 14, Issue 1. 16 February 2014. Article number 7
DOI
10.1186/1471-213X-14-7