Electroablation: A method for neurectomy and localized tissue injury

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dc.contributor.authorMoya-Díaz, José
dc.contributor.authorPeña, Oscar A.
dc.contributor.authorSánchez, Mario
dc.contributor.authorUreta, Daniela A
dc.contributor.authorReynaert, Nicole G
dc.contributor.authorAnguita-Salinas, Consuelo
dc.contributor.authorMarín, Gonzalo
dc.contributor.authorAllende, Miguel L
dc.date.accessioned2023-06-27T15:22:53Z
dc.date.available2023-06-27T15:22:53Z
dc.date.issued2014-02
dc.descriptionIndexación: Scopus.es
dc.description.abstractAbstract Background: Tissue injury has been employed to study diverse biological processes such as regeneration and inflammation. In addition to physical or surgical based methods for tissue injury, current protocols for localized tissue damage include laser and two-photon wounding, which allow a high degree of accuracy, but are expensive and difficult to apply. In contrast, electrical injury is a simple and inexpensive technique, which allows reproducible and localized cell or tissue damage in a variety of contexts. Results: We describe a novel technique that combines the advantages of zebrafish for in vivo visualization of cells with those of electrical injury methods in a simple and versatile protocol which allows the study of regeneration and inflammation. The source of the electrical pulse is a microelectrode that can be placed with precision adjacent to specific cells expressing fluorescent proteins. We demonstrate the use of this technique in zebrafish larvae by damaging different cell types and structures. Neurectomy can be carried out in peripheral nerves or in the spinal cord allowing the study of degeneration and regeneration of nerve fibers. We also apply this method for the ablation of single lateral line mechanosensory neuromasts, showing the utility of this approach as a tool for the study of organ regeneration. In addition, we show that electrical injury induces immune cell recruitment to damaged tissues, allowing in vivo studies of leukocyte dynamics during inflammation within a confined and localized injury. Finally, we show that it is possible to apply electroablation as a method of tissue injury and inflammation induction in adult fish. Conclusions: Electrical injury using a fine microelectrode can be used for axotomy of neurons, as a general tissue ablation tool and as a method to induce a powerful inflammatory response. We demonstrate its utility to studies in both larvae and in adult zebrafish but we expect that this technique can be readily applied to other organisms as well. We have called this method of electrical based tissue ablation, electroablation.es
dc.description.urihttps://bmcdevbiol.biomedcentral.com/articles/10.1186/1471-213X-14-7#citeas
dc.identifier.citationBMC Developmental Biology. Volume 14, Issue 1. 16 February 2014. Article number 7es
dc.identifier.doi10.1186/1471-213X-14-7
dc.identifier.issn1471-213X
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/51082
dc.language.isoenes
dc.publisherBMCes
dc.rights.licenseAtribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectAxotomyes
dc.subjectNeurectomyes
dc.subjectTissue Ablationes
dc.subjectRegenerationes
dc.subjectInflammationes
dc.subjectZebrafishes
dc.titleElectroablation: A method for neurectomy and localized tissue injuryes
dc.typeArtículoes
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