Examinando por Autor "Sallaberry-Pincheira, Nicole"
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Ítem First confirmed report of Hawksbill Sea Turtle Eretmochelys imbricata in nearshore waters of Easter Island (Rapa Nui)(Universidad de Valparaíso. Facultad de Ciencias del Mar, 2015) Álvarez-Varas, Rocío; Flores, Marcelo; Demangel, Diego; García, Michel; Sallaberry-Pincheira, NicoleThe hawksbill turtle (Eretmochelys imbricata; Critically Endangered) has a circumtropical distribution. In Polynesia it inhabits the waters of a great number of islands; however, up to date there are no official records for Easter Island. We document the first report of E. imbricata in Easter Island and Chile based on underwater photographs and examination of one individual. The confirmation of the presence of hawksbills extends the known distribution range for the species, increases the number of sea turtle species recorded for Chile, and highlights the need for further research on potential threats to the species and the importance of Rapa Nui as developmental and foraging habitat for hawksbills.Ítem How to perform an electrocardiogram in an awake pond slider turtle (Trachemys scripta): a comparative study of five noninvasive methodologies(American journal of veterinary research, Volume 85, Issue 111 November 2024, 2024-11) Hammond, Hillary K.; Sallaberry-Pincheira, Nicole; Santangelo, Stephen; Barnett, Brian G.; Divers, Stephen J.OBJECTIVE: To compare multiple noninvasive ECG methods in pond sliders based upon repeatability, ability to recognize standard waveforms, and measurability. METHODS: The study was performed from November 2023 through January 2024. Ten healthy adult pond turtles were enrolled in the study. ECG tracings were obtained using 4 previously reported and 1 novel ECG methodology, using adhesive patches applied to the prehumeral fossae and abdominal scutes. The 50 ECG tracings were blinded by method and turtle, randomized for evaluation by 4 observers, and assessed for quality on a scale from 0 to 3. RESULTS: Interobserver and intraobserver intraclass correlation coefficients for all tracings were 0.84 and 0.97, respectively, indicating an almost perfect agreement. The average score amongst the observers for each tracing was then averaged by method, ranging from 0.875 to 2.15. The novel method demonstrated a collective average of 2.15 and was the highest scoring method for 8 of 10 turtles. CONCLUSIONS: Electrocardiogram utilizing methods that apply adhesive patches to the prehumeral fossae and either the abdominal scutes of the plastron or prefemoral fossae in pond turtles can be performed to produce recognizable waveforms. CLINICAL RELEVANCE: Diagnostic tools, such as ECGs, are imperative to enhance veterinary care in nonconventional species, particularly with the rising trend of exotic pets worldwide.Ítem Large-scale survey for canine vector-borne parasites in free-ranging dogs and foxes from six diverse bioclimatic regions of Chile(Elsevier B.V., 2022-05) Di Cataldo, Sophia; Cevidanes, Aitor; Ulloa-Contreras, Claudia; Cabello, Javier; Gambino, Delia; Gargano, Valeria; Hidalgo-Hermoso, Ezequiel; Latrofa, Maria Stefania; Napolitano, Constanza; Sacristán, Irene; Sallaberry-Pincheira, Nicole; Vianna, Juliana; Acosta-Jamett, Gerardo; Vicari, Domenico; Otranto, Domenico; Millán, JavierChile is a large country with a marked range of climate conditions that make it an ideal scenario for the study of vector-borne parasites (VBPs); however, knowledge about their distribution is limited to a few confined areas of this country. The presence of Hepatozoon spp., piroplasmids, Leishmania spp. and filarioids was investigated through molecular and serological methods in blood and serum samples of 764 free-ranging rural dogs, 154 Andean foxes (Lycalopex culpaeus), and 91 South American grey foxes (Lycalopex griseus) from six bioclimatic regions across Chile. Hepatozoon spp. DNA was exclusively detected in foxes (43% prevalence), including sequences closely related to Hepatozoon felis (24.1%; only Andean foxes), Hepatozoon americanum (16.2%; only grey foxes), and Hepatozoon canis (1.25%; in one grey fox). Risk factor assessment identified a higher probability of Hepatozoon infection in juvenile foxes. DNA of piroplasmids was detected in 0.7% of dogs (Babesia vogeli) but in no fox, whilst antibodies against Babesia sp. were detected in 24% of the dogs and 25% of the foxes, suggesting a wider circulation of canine piroplasmids than previously believed. A positive association between the presence of antibodies against Babesia and high Rhipicephalus sanguineus sensu lato burden was observed in dogs. Leishmania spp. DNA and antibodies were detected in 0.8% and 4.4% of the dogs, respectively. Acanthocheilonema reconditum was the only blood nematode detected (1.5% of the dogs and no fox). Differences in prevalence among bioregions were observed for some of the VBPs. These results expand our knowledge about the occurrence of vector-borne parasites in Chile, some of which are firstly reported herein. This information will facilitate the diagnosis of vector-borne diseases in domestic dogs and improve the control measures for both domestic and wild canids. © 2022 Elsevier B.V.Ítem Molecular identification of Bartonella spp. and Rickettsia felis in fox fleas, Chile(Elsevier Ltd, 2023-05) Millán, Javier; Sepúlveda-García, Paulina; Di Cataldo, Sophia; Canales, Nivia; Sallaberry-Pincheira, Nicole; Painean, Javier; Cevidanes, Aitor; Müller, AnandaSeventy-five flea pools (one to ten fleas per pool) from 51 Andean foxes (Lycalopex culpaeus) and five South American grey foxes or chillas (Lycalopex griseus) from the Mediterranean region of Chile were analyzed for the presence of DNA of Bartonella spp. and Rickettsia spp. through quantitative real-time PCR for the nouG and gltA genes, respectively. Positive samples were further characterized by conventional PCR protocols, targeting gltA and ITS genes for Bartonella, and gltA, ompA, and ompB genes for Rickettsia. Bartonella was detected in 48 % of the Pulex irritans pools (B. rochalimae in three pools, B. berkhoffii in two pools, B. henselae in one pool), and 8 % of the Ctenocephalides felis felis pools (B. rochalimae, one pool). Rickettsia was confirmed in 11 % of P. irritans pools and 92 % of the Ct. felis pools. Characterization confirmed R. felis in all sequenced Rickettsia-positive pools. All Ct. canis pools were negative. A Ct. felis pool from a wild-found domestic ferret (Mustela putorius furo) also resulted positive for R. felis. Although opportunistic, this survey provides the first description of zoonotic pathogens naturally circulating in fleas parasitizing Chilean free-living carnivores. © 2023 The AuthorsÍtem Molecular surveillance of potential SARS-CoV-2 reservoir hosts in wildlife rehabilitation centers(Veterinary Quarterly, Volume 43, Issue 1, Pages 1 - 102023, 2023) Mena, Juan; Hidalgo, Christian; Estay-Olea, Daniela; Sallaberry-Pincheira, Nicole; Bacigalupo, Antonella; Rubio, André V.; Peñaloza, Diego; Sánchez, Carolina; Gómez-Adaros, Javiera; Olmos, Valeria; Cabello, Javier; Ivelic, Kendra; Abarca, María José; Ramírez-Álvarez, Diego; Torregrosa Rocabado, Marisol; Durán Castro, Natalia; Carreño, Martina; Gómez, Gabriela; Cattan, Pedro E.; Ramírez-Toloza, Galia; Robbiano, Sofía; Marchese, Carla; Raffo, Eduardo; Stowhas, Paulina; Medina-Vogel, Gonzalo; Landaeta-Aqueveque, Carlos; Ortega, René; Waleckx, Etienne; Gónzalez-Acuña, Daniel; Rojo, GemmaBackground: The COVID-19 pandemic, caused by SARS-CoV-2 infection, has become the most devastating zoonotic event in recent times, with negative impacts on both human and animal welfare as well as on the global economy. Although SARS-CoV-2 is considered a human virus, it likely emerged from animals, and it can infect both domestic and wild animals. This constitutes a risk for human and animal health including wildlife with evidence of SARS-CoV-2 horizontal transmission back and forth between humans and wild animals. Aim: Molecular surveillance in different wildlife rehabilitation centers and wildlife associated institutions in Chile, which are critical points of animal-human interaction and wildlife conservation, especially since the aim of wildlife rehabilitation centers is to reintroduce animals to their original habitat. Materials and Methods: The survey was conducted in six WRCs and three wildlife associated institutions. A total of 185 samples were obtained from 83 individuals belonging to 15 different species, including vulnerable and endangered species. Each specimen was sampled with two different swabs: one oropharyngeal or nasopharyngeal according to the nostril diameter, and/or a second rectal sample. RNA was extracted from the samples and two different molecular assays were performed: first, a conventional RT-PCR with pan-coronavirus primers and a second SARS-CoV-2 qPCR targeting the N and S genes. Results: All 185 samples were negative for SARS-CoV-2. Clinical relevance: This study constitutes the first report on the surveillance of SARS-CoV-2 from wildlife treated in rehabilitation centers in Chile, and supports the biosafety procedures adopted in those centers. © 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group