Molecular surveillance of potential SARS-CoV-2 reservoir hosts in wildlife rehabilitation centers
No hay miniatura disponible
Archivos
Fecha
2023
Profesor/a Guía
Facultad/escuela
Idioma
en_US
Título de la revista
ISSN de la revista
Título del volumen
Editor
Veterinary Quarterly, Volume 43, Issue 1, Pages 1 - 102023
Nombre de Curso
Licencia CC
Attribution 4.0 International
CC BY 4.0
Deed
Licencia CC
https://creativecommons.org/licenses/by/4.0/
Resumen
Background: The COVID-19 pandemic, caused by SARS-CoV-2 infection, has become the most devastating zoonotic event in recent times, with negative impacts on both human and animal welfare as well as on the global economy. Although SARS-CoV-2 is considered a human virus, it likely emerged from animals, and it can infect both domestic and wild animals. This constitutes a risk for human and animal health including wildlife with evidence of SARS-CoV-2 horizontal transmission back and forth between humans and wild animals. Aim: Molecular surveillance in different wildlife rehabilitation centers and wildlife associated institutions in Chile, which are critical points of animal-human interaction and wildlife conservation, especially since the aim of wildlife rehabilitation centers is to reintroduce animals to their original habitat. Materials and Methods: The survey was conducted in six WRCs and three wildlife associated institutions. A total of 185 samples were obtained from 83 individuals belonging to 15 different species, including vulnerable and endangered species. Each specimen was sampled with two different swabs: one oropharyngeal or nasopharyngeal according to the nostril diameter, and/or a second rectal sample. RNA was extracted from the samples and two different molecular assays were performed: first, a conventional RT-PCR with pan-coronavirus primers and a second SARS-CoV-2 qPCR targeting the N and S genes. Results: All 185 samples were negative for SARS-CoV-2. Clinical relevance: This study constitutes the first report on the surveillance of SARS-CoV-2 from wildlife treated in rehabilitation centers in Chile, and supports the biosafety procedures adopted in those centers. © 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group
Notas
Indexación: Scopus.
Palabras clave
Animals, Animals, Wild, COVID-19, Humans, Pandemics, SARS-CoV-2 EMTREE, amino acid sequence, animal experiment, animal model, animal welfare, controlled study, coronavirus disease 2019, disease surveillance, female, fluorometry, male, nasopharyngeal swab, nonhuman, oropharyngeal swab, real time polymerase chain reaction, rehabilitation center, reverse transcription polymerase chain reaction, RNA extraction, RNA isolation, Severe acute respiratory syndrome coronavirus 2, wildlife, epidemiology, veterinary medicine
Citación
DOI
10.1080/01652176.2023.2164909