Molecular surveillance of potential SARS-CoV-2 reservoir hosts in wildlife rehabilitation centers

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dc.contributor.authorMena, Juan
dc.contributor.authorHidalgo, Christian
dc.contributor.authorEstay-Olea, Daniela
dc.contributor.authorSallaberry-Pincheira, Nicole
dc.contributor.authorBacigalupo, Antonella
dc.contributor.authorRubio, André V.
dc.contributor.authorPeñaloza, Diego
dc.contributor.authorSánchez, Carolina
dc.contributor.authorGómez-Adaros, Javiera
dc.contributor.authorOlmos, Valeria
dc.contributor.authorCabello, Javier
dc.contributor.authorIvelic, Kendra
dc.contributor.authorAbarca, María José
dc.contributor.authorRamírez-Álvarez, Diego
dc.contributor.authorTorregrosa Rocabado, Marisol
dc.contributor.authorDurán Castro, Natalia
dc.contributor.authorCarreño, Martina
dc.contributor.authorGómez, Gabriela
dc.contributor.authorCattan, Pedro E.
dc.contributor.authorRamírez-Toloza, Galia
dc.contributor.authorRobbiano, Sofía
dc.contributor.authorMarchese, Carla
dc.contributor.authorRaffo, Eduardo
dc.contributor.authorStowhas, Paulina
dc.contributor.authorMedina-Vogel, Gonzalo
dc.contributor.authorLandaeta-Aqueveque, Carlos
dc.contributor.authorOrtega, René
dc.contributor.authorWaleckx, Etienne
dc.contributor.authorGónzalez-Acuña, Daniel
dc.contributor.authorRojo, Gemma
dc.date.accessioned2025-03-10T14:56:44Z
dc.date.available2025-03-10T14:56:44Z
dc.date.issued2023
dc.descriptionIndexación: Scopus.
dc.description.abstractBackground: The COVID-19 pandemic, caused by SARS-CoV-2 infection, has become the most devastating zoonotic event in recent times, with negative impacts on both human and animal welfare as well as on the global economy. Although SARS-CoV-2 is considered a human virus, it likely emerged from animals, and it can infect both domestic and wild animals. This constitutes a risk for human and animal health including wildlife with evidence of SARS-CoV-2 horizontal transmission back and forth between humans and wild animals. Aim: Molecular surveillance in different wildlife rehabilitation centers and wildlife associated institutions in Chile, which are critical points of animal-human interaction and wildlife conservation, especially since the aim of wildlife rehabilitation centers is to reintroduce animals to their original habitat. Materials and Methods: The survey was conducted in six WRCs and three wildlife associated institutions. A total of 185 samples were obtained from 83 individuals belonging to 15 different species, including vulnerable and endangered species. Each specimen was sampled with two different swabs: one oropharyngeal or nasopharyngeal according to the nostril diameter, and/or a second rectal sample. RNA was extracted from the samples and two different molecular assays were performed: first, a conventional RT-PCR with pan-coronavirus primers and a second SARS-CoV-2 qPCR targeting the N and S genes. Results: All 185 samples were negative for SARS-CoV-2. Clinical relevance: This study constitutes the first report on the surveillance of SARS-CoV-2 from wildlife treated in rehabilitation centers in Chile, and supports the biosafety procedures adopted in those centers. © 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group
dc.description.urihttps://www-tandfonline-com.recursosbiblioteca.unab.cl/doi/full/10.1080/01652176.2023.2164909#d1e771
dc.identifier.doi10.1080/01652176.2023.2164909
dc.identifier.issn01652176
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/63704
dc.language.isoen_US
dc.publisherVeterinary Quarterly, Volume 43, Issue 1, Pages 1 - 102023
dc.rights.licenseAttribution 4.0 International CC BY 4.0 Deed
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAnimals
dc.subjectAnimals, Wild
dc.subjectCOVID-19
dc.subjectHumans
dc.subjectPandemics
dc.subjectSARS-CoV-2 EMTREE
dc.subjectamino acid sequence
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal welfare
dc.subjectcontrolled study
dc.subjectcoronavirus disease 2019
dc.subjectdisease surveillance
dc.subjectfemale
dc.subjectfluorometry
dc.subjectmale
dc.subjectnasopharyngeal swab
dc.subjectnonhuman
dc.subjectoropharyngeal swab
dc.subjectreal time polymerase chain reaction
dc.subjectrehabilitation center
dc.subjectreverse transcription polymerase chain reaction
dc.subjectRNA extraction
dc.subjectRNA isolation
dc.subjectSevere acute respiratory syndrome coronavirus 2
dc.subjectwildlife
dc.subjectepidemiology
dc.subjectveterinary medicine
dc.titleMolecular surveillance of potential SARS-CoV-2 reservoir hosts in wildlife rehabilitation centers
dc.typeArtículo
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