Examinando por Autor "Sepulveda, Beatriz"
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Ítem Are Ionic Liquids Better Extracting Agents Than Toxic Volatile Organic Solvents? A Combination of Ionic Liquids, Microwave and LC/MS/MS, Applied to the Lichen Stereocaulon glareosum(Frontiers Media S.A., 2020-05) Calla Quispe, Erika; Robles, Juana; Areche, Carlos; Sepulveda, BeatrizWe report a green strategy for the extraction of lichen substances from Stereocaulon glareosum. This sustainable alternative does not use volatile toxic organic solvents, but it is assisted by microwave and is checked by UHPLC/ESI/MS/MS. Ionic liquids may provide a better alternative in the extraction of natural products from lichens. © Copyright © 2020 Calla-Quispe, Robles, Areche and Sepulveda.Ítem Corryocactus brevistylus (K. Schum. ex Vaupel) Britton & Rose (Cactaceae): Antioxidant, Gastroprotective Effects, and Metabolomic Profiling by Ultrahigh-Pressure Liquid Chromatography and Electrospray High Resolution Orbitrap Tandem Mass Spectrometry(Frontiers Media S.A., 2022-04) Areche, Carlos; Hernandez, Marco; Cano, Teresa; Ticona, Juana; Cortes, Carmen; Simirgiotis, Mario; Caceres, Fátima; Borquez, Jorge; Echeverría, Javier; Sepulveda, BeatrizCorryocactus brevistylus (K. Schum. ex Vaupel) Britton & Rose (Cactaceae) is a shrubby or often arborescent cactus popularly known as “sancayo” and produce an edible fruit known as “Sanky” which is consumed in Arequipa-Perú. The purpose of this study was to report the gastroprotective activity and relate this activity to the antioxidant capacity and presence of phenolic compounds for the first time. A metabolomic profiling based on Ultrahigh-pressure liquid chromatography and electrospray high resolution mass spectrometry, and the antioxidant activities (DPPH, ABTS, and FRAP), ascorbic acid content, total phenolics and flavonoids contents, and the mode of gastroprotective action of the Sanky fruit including the involvement of prostaglandins, nitric oxide, and sulfhydryl compounds is reported. Thirty-eight compounds were detected in the ethanolic extract including 12 organic acids, nine hydroxycinnamic acids, three isoamericanol derivatives, six flavonoids, five fatty acids, and two sterols. The results of the biological tests showed that the ethanolic extract had antioxidant capacity and gastroprotective activity on the model of HCl/EtOH-induced gastric lesions in mice (at 10, 25, 50, and 100 mg/kg). The effect elicited by the extract at 50 mg/kg was reversed by indometacin and N-ethylmaleimide but not by NG-nitro-L-arginine methyl ester suggesting that sulfhydryl groups and prostaglandins are involved in the mode of gastroprotective action. In conclusion, our study proves that C. brevistylus pears have some gastroprotective and antioxidant capacities and consumption is recommended for the presence of several bioactive compounds. © Copyright © 2020 Areche, Hernandez, Cano, Ticona, Cortes, Simirgiotis, Caceres, Borquez, Echeverría and Sepulveda.Ítem Lichens: might be considered as a source of gastroprotective molecules?(2013) Sepulveda, Beatriz; Chamy, María Cristina; Piovano, Marisa; Areche, CarlosABSTRACT Lichens are symbiotic relationship between fungi and algae or cyanobacteria. Secondary metabolites from lichens are known as lichen substances. We investigated depsidone and depside from lichens 1-6 in the context of their action to prevent gastric ulcer on the model of HCl/ethanol in mice for the first time. Doses of 30 mg/kg of lichen substances 1-6 and positive control (lansoprazole) significantly diminished the lesion index compared with negative control (treated only with HCl/EtOH). Lobaric acid 1, atranorin 2 and psoromic acid 5 reduced the gastric lesions by 76%, 63% and 65%, while for variolaric acid 3, diffractaic acid 4 and perlatolic acid 6 their values were 32%, 14% and 45%, respectively. Our results suggest that lichens have potential as a suite of gastroprotective molecules. Keywords: Lichens; depside; depsidone; gastric ulcer;Ítem Microwave-Assisted Extraction of Secondary Metabolites Using Ethyl Lactate Green Solvent from Ambrosia arborescens: LC/ESI-MS/MS and Antioxidant Activity(Multidisciplinary Digital Publishing Institute (MDPI), 2024-05) Guillen, Evelyn; Terrones, Hector; de Terrones, Teresa Cano; Simirgiotis, Mario J.; Hájek, Jan; Cheel, José; Sepulveda, Beatriz; Areche, CarlosAlternative solvents are being tested as green solvents to replace the traditional organic solvents used in both academy and industry. Some of these are already available, such as ethyl lactate, cyrene, limonene, glycerol, and others. This alternative explores eco-friendly processes for extracting secondary metabolites from nature, thus increasing the number of unconventional extraction methods with lower environmental impact over conventional methods. In this context, the Peruvian Ambrosia arborescens was our model while exploring a microwave-assisted extraction (MAE) approach over maceration. The objective of this study was to perform a phytochemical study including UHPLC-ESI-MS/MS and the antioxidant activity of Ambrosia arborescens, using sustainable strategies by mixing both microwaves and ethyl lactate as a green solvent. The results showed that ethyl lactate/MAE (15.07%) achieved a higher extraction yield than methanol/maceration (12.6%). In the case of the isolation of psilostachyin, it was similar to ethyl lactate (0.44%) when compared to methanol (0.40%). Regarding UHPLC-ESI-MS/MS studies, the results were similar. Twenty-eight compounds were identified in the ethyl lactate/MAE and methanol/maceration extracts, except for the tentative identification of two additional amino acids (peaks 4 and 6) in the MeOH extract. In relation to the antioxidant assay, the activity of the ethyl lactate extract was a little higher than the methanol extract in terms of ORAC (715.38 ± 3.2) and DPPH (263.04 ± 2.8). This study on A. arborescens demonstrated that the unconventional techniques, such as MAE related to ethyl lactate, could replace maceration/MeOH for the extraction and isolation of metabolites from diverse sources. This finding showed the potential of unconventional methods with green solvents to provide eco-friendly methods based on green chemistry.Ítem Mulinum crassifolium phil; Two new mulinanes, gastroprotective activity and metabolomic analysis by uhplc-orbitrap mass spectrometry(MDPI, 2019-04) Areche, Carlos; Fernandez-Burgos, Ronald; Cano De Terrones, Teresa; Simirgiotis, Mario; García-Beltrán, Olimpo; Borquez, Jorge; Sepulveda, BeatrizMulinum crassifolium Phil. (Apiaceae) is an endemic shrub from Chile commonly used as infusion in traditional medicine to treat diabetes, bronchial and intestinal disorders and stomach ailments, including ulcers. From the EtOAc extract of this plant, the new mulinane-type diterpenoids 3 and 5 were isolated along with three known diterpenoids. The gastroprotective effect of the infusion of the plant was assayed to support the traditional use and a fast HPLC analysis using high resolution techniques was performed to identify the bioactive constituents. The EtOAc extract and the edible infusion showed gastroprotective effect at 100 mg/kg in the HCl/EtOH induced gastric ulcer model in mice, reducing lesions by 33% and 74%, respectively. Finally, a metabolomic profiling based on UHPLC-ESI-MS/HRMS of the edible infusion was performed and thirty-five compounds were tentatively identified including quercetin, caffeic acid, apigenine glucoside, p-coumaric acid, chlorogenic acids, and caffeoylquinic acids, which have been associated previously with gastroprotective and antiulcer properties. This scientific evidence can support the contribution of polyphenols in the gastroprotective activity of the edible infusion of this plant, and can validate at least in part, its ethnopharmacological use. © 2019 by the authors.Ítem UHPLC-MS Metabolomic Fingerprinting, Antioxidant, and Enzyme Inhibition Activities of Himantormia lugubris from Antarctica(MDPI, 2022-06) Areche, Carlos; Parra, Javier Romero; Sepulveda, Beatriz; García-Beltrán, Olimpo; Simirgiotis, Mario J.Himantormia lugubris is a Chilean native small lichen shrub growing in the Antarctica re-gion. In this study, the metabolite fingerprinting and the antioxidant and enzyme inhibitory potential from this species and its four major isolated compounds were investigated for the first time. Using ultra-high performance liquid chromatography coupled to quadrupole-Orbitrap mass spec-trometry analysis (UHPLC-Q-Orbitrap-MS), several metabolites were identified including specific compounds as chemotaxonomical markers, while major metabolites were quantified in this species. A good inhibition activity against cholinesterase (acetylcholinesterase (AChE) IC50: 12.38 ± 0.09 µg/mL, butyrylcholinesterase (BChE) IC50: 31.54 ± 0.20 µg/mL) and tyrosinase (22.32 ± 0.21 µg/mL) enzymes of the alcoholic extract and the main compounds (IC50: 28.82 ± 0.10 µg/mL, 36.43 ± 0.08 µg/mL, and 7.25 ± 0.18 µg/mL, respectively, for the most active phenolic atranol) was found. The extract showed a total phenolic content of 47.4 + 0.0 mg of gallic acid equivalents/g. In addition, antioxidant activity was assessed using bleaching of DPPH and ORAC (IC50: 75.3 ± 0.02 µg/mL and 32.7 ± 0.7 μmol Trolox/g lichen, respectively) and FRAP (27.8 ± 0.0 μmol Trolox equivalent/g) ex-periments. The findings suggest that H. lugubris is a rich source of bioactive compounds with po-tentiality in the prevention of neurodegenerative or noncommunicable chronic diseases. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.Ítem Untargeted Metabolomics by Using UHPLC–ESI–MS/MS of an Extract Obtained with Ethyl Lactate Green Solvent from Salvia rosmarinus(MDPI, 2022-11) Castañeta, Grover; Cifuentes, Nicolas; Sepulveda, Beatriz; Bárcenas Pérez, Daniela; Cheel, José; Areche, CarlosSalvia rosmarinus (Lamiaceae), previously known as Rosmarinus officinalis, is a plant cultivated worldwide, native to the Mediterranean region. Its leaves are traditionally used for cooking. This species possesses numerous biological activities, including antioxidant, antimicrobial, anticancer, anti-inflammatory, and hepatoprotective properties. These biological properties are due to the presence of phenolic compounds, including rosmarinic acid and phenolic diterpenoids, such as carnosic acid and carnosol. In this study, we investigated the chemical composition of a green extract obtained by maceration with ethyl lactate for the first time. Seventy-five compounds were tentatively identified by UHPLC–ESI–MS/MS, including six organic acids, six cinnamic acid derivatives, five fatty acids, eighteen flavonoids, and thirty-eight terpenoids. Thus, abietane-type diterpenoids from the ethyl lactate extract were the predominant diterpenoids in the Chilean S. rosmarinus species, in contrast to the Chinese species, in which labdane and isopimarane-type diterpenoids were found for the first time. Finally, our study confirms that the extraction of S. rosmarinus with green ethyl lactate as a solvent is efficient and sustainable for the identification of flavonoids, phenols, and terpenoids from leaves. © 2022 by the authors.