Examinando por Autor "Valdés, Jorge H."

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    Draft genome of Pseudomonas sp. RGM 2987 isolated from Stevia philippiana roots reveals its potential as a plant biostimulant and potentially constitutes a novel species
    (Pontificia Universidad Catolica de Valparaiso, 2023-01) Guerra, Matías; Carrasco-Fernández, Jorge; Valdés, Jorge H.; Panichini, Marcelo; Castro, Jean Franco
    Background: Reports on Pseudomonas species associated with Chilean native plants and their diversity are scarce. The draft genome of Pseudomonas sp. RGM 2987 isolated from the rhizosphere of Stevia philippiana, a native Chilean plant from the Altiplano, is presented. Results: The assembled genome features 6,161,133 bp, a G+C content of 61.3%, and 5,350 predicted open reading frames. dDDH, and ANIb differences between RGM 2987 and the closest relatives support its classification as a new species within the Pseudomonas genus. Genome mining and functional classification revealed the presence of genes involved in cell function and metabolism as well as plant-growth promotion, including those for indole acetic acid production, phosphate solubilization, and ethylene concentration. Conclusions: The draft genome of Pseudomonas sp. RGM 2987 provides insights on its phylogeny and classification as a new species and shed light on its potential as a plant-biostimulant, expanding our knowledge on Pseudomonas biodiversity worldwide and, specifically, for those strains associated with native Chilean plants. How to cite: Guerra M, Carrasco-Fernández J, Valdés JH, et al. Draft genome of Pseudomonas sp. RGM 2987 isolated from Stevia philippiana roots reveals its potential as a plant biostimulant and potentially constitutes a novel species. Electron J Biotechnol 2022;61. https://doi-org.recursosbiblioteca.unab.cl/10.1016/j.ejbt.2022.10.001. © 2022
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    Draft Genome Sequence of Pseudomonas sp. Strain RGM 3321, a Phyllosphere Endophyte from Fragaria chiloensis subsp. chiloensis f. patagonica
    (American Society for Microbiology, 2022-07) Castro, Jean Franco; Guerra, Matías; Carrasco Fernández, Jorge; Ortiz Campos, Javiera; Cares Gatica, Diego; Campos Quiroz, Carolina; Correa, Francisco; Beltrán, M. Francisca; Sagredo, Boris; Valdés, Jorge H.
    Pseudomonas sp. strain RGM 3321 is a phyllosphere endophyte from Fragaria chiloensis subsp. chiloensis f. patagonica that harbors genes associated with plant growth promotion pathways, as well as genes typically found in plant pathogens. © 2022 Castro et al.
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    Integrative Genomics Sheds Light on Evolutionary Forces Shaping the Acidithiobacillia Class Acidophilic Lifestyle
    (Frontiers Media S.A., 2022-02-15) González-Rosales, Carolina; Vergara, Eva; Dopson, Mark; Valdés, Jorge H.; Holmes, David S.
    Extreme acidophiles thrive in environments rich in protons (pH values <3) and often high levels of dissolved heavy metals. They are distributed across the three domains of the Tree of Life including members of the Proteobacteria. The Acidithiobacillia class is formed by the neutrophilic genus Thermithiobacillus along with the extremely acidophilic genera Fervidacidithiobacillus, Igneacidithiobacillus, Ambacidithiobacillus, and Acidithiobacillus. Phylogenomic reconstruction revealed a division in the Acidithiobacillia class correlating with the different pH optima that suggested that the acidophilic genera evolved from an ancestral neutrophile within the Acidithiobacillia. Genes and mechanisms denominated as “first line of defense” were key to explaining the Acidithiobacillia acidophilic lifestyle including preventing proton influx that allows the cell to maintain a near-neutral cytoplasmic pH and differ from the neutrophilic Acidithiobacillia ancestors that lacked these systems. Additional differences between the neutrophilic and acidophilic Acidithiobacillia included the higher number of gene copies in the acidophilic genera coding for “second line of defense” systems that neutralize and/or expel protons from cell. Gain of genes such as hopanoid biosynthesis involved in membrane stabilization at low pH and the functional redundancy for generating an internal positive membrane potential revealed the transition from neutrophilic properties to a new acidophilic lifestyle by shaping the Acidithiobacillaceae genomic structure. The presence of a pool of accessory genes with functional redundancy provides the opportunity to “hedge bet” in rapidly changing acidic environments. Although a core of mechanisms for acid resistance was inherited vertically from an inferred neutrophilic ancestor, the majority of mechanisms, especially those potentially involved in resistance to extremely low pH, were obtained from other extreme acidophiles by horizontal gene transfer (HGT) events. Copyright © 2022 González-Rosales, Vergara, Dopson, Valdés and Holmes.