Enhanced Glutathione Content Allows the In Vivo Synthesis of Fluorescent CdTe Nanoparticles by Escherichia coli
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Fecha
2012-11-21
Profesor/a Guía
Facultad/escuela
Idioma
en
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Editor
Public Library of Science
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Licencia CC
CC BY 4.0 DEED
Atribución 4.0 Internacional
https://creativecommons.org/licenses/by/4.0/deed.es
https://creativecommons.org/licenses/by/4.0/deed.es
Licencia CC
Resumen
The vast application of fluorescent semiconductor nanoparticles (NPs) or quantum dots (QDs) has prompted the development of new, cheap and safer methods that allow generating QDs with improved biocompatibility. In this context, green or biological QDs production represents a still unexplored area. This work reports the intracellular CdTe QDs biosynthesis in bacteria. Escherichia coli overexpressing the gshA gene, involved in glutathione (GSH) biosynthesis, was used to produce CdTe QDs. Cells exhibited higher reduced thiols, GSH and Cd/Te contents that allow generating fluorescent intracellular NP-like structures when exposed to CdCl2 and K2TeO3. Fluorescence microscopy revealed that QDs-producing cells accumulate defined structures of various colors, suggesting the production of differently-sized NPs. Purified fluorescent NPs exhibited structural and spectroscopic properties characteristic of CdTe QDs, as size and absorption/emission spectra. Elemental analysis confirmed that biosynthesized QDs were formed by Cd and Te with Cd/Te ratios expected for CdTe QDs. Finally, fluorescent properties of QDs-producing cells, such as color and intensity, were improved by temperature control and the use of reducing buffers. © 2012 Monrás et al.
Notas
INDEXACIÓN: SCOPUS.
Palabras clave
Cadmium Compounds, Citrates, Escherichia coli, Genes, Bacterial, Glutathione, Microscopy, Fluorescence, Nanoparticles, Particle Size, Quantum Dots, Spectrometry, Fluorescence, Spectroscopy, Fourier Transform Infrared, Tellurium, Temperature, X-Ray Diffraction
Citación
PLoS ONE, Volume 7, Issue 11, 21 November 2012, Article number e48657
DOI
10.1371/journal.pone.0048657