Lipopolysaccharide from Porphyromonas gingivalis, but Not from Porphyromonas endodontalis, Induces Macrophage M1 Profile

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dc.contributor.authorVeloso, Pablo
dc.contributor.authorFernández, Alejandra
dc.contributor.authorAstorga, Jessica
dc.contributor.authorGonzález Quintanilla, David
dc.contributor.authorCastro, Alfredo
dc.contributor.authorEscobar, Alejandro
dc.contributor.authorHoare, Anilei
dc.contributor.authorHernández, Marcela
dc.date.accessioned2023-09-25T15:38:45Z
dc.date.available2023-09-25T15:38:45Z
dc.date.issued2022-09
dc.descriptionIndexación: Scopus.es
dc.description.abstractApical Lesions of Endodontic Origin (ALEO) are initiated by polymicrobial endodontic canal infection. Porphyromonas gingivalis (Pg) and Porphyromonas endodontalis (Pe) lipopolysaccharides (LPS) can induce a pro-inflammatory macrophage response through their recognition by TLR2 and TLR4. However, polarization responses induced by Pg and/or Pe LPS in macrophages are not fully understood. We aimed to characterize the polarization profiles of macrophages differentiated from THP-1 cells following Pg and/or Pe LPS stimulation from reference strain and clinical isolates. A modified LPS purification protocol was implemented and the electrophoretic LPS profiles were characterized. THP-1 human monocytes differentiated to macrophages were stimulated with Pg and Pe LPS. Polarization profiles were characterized through cell surface markers and secreted cytokines levels after 24 h of stimulation. TLR2 and TLR4 cell surfaces and transcriptional levels were determined after 24 or 2 h of LPS stimulation, respectively. LPS from Pg induced a predominant M1 profile in macrophages evidenced by changes in the expression of the surface marker CD64 and pro-inflammatory cytokine profiles, TNF-α, IL-1β, IL-6, and IL-12. Pe LPS was unable to induce a significant response. TLR2 and TLR4 expressions were neither modified by Pg or Pe LPS. Pg LPS, but not Pe LPS, induced a macrophage M1 Profile. © 2022 by the authors.es
dc.description.urihttps://www.mdpi.com/1422-0067/23/17/10011
dc.identifier.citationInternational Journal of Molecular Sciences, Volume 23, Issue 17, September 2022, Article number 10011es
dc.identifier.doi10.3390/ijms231710011
dc.identifier.issn1661-6596
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/53299
dc.language.isoenes
dc.publisherMDPIes
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectLipopolysaccharidees
dc.subjectMacrophage polarizationes
dc.subjectPorphyromonas endodontalises
dc.subjectPorphyromonas gingivalises
dc.subjectToll-like receptores
dc.titleLipopolysaccharide from Porphyromonas gingivalis, but Not from Porphyromonas endodontalis, Induces Macrophage M1 Profilees
dc.typeArtículoes
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