The cotranscribed Salmonella enterica sv. Typhi Tsx and impX genes encode opposing nucleoside-specific import and export proteins

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dc.contributor.authorBucarey, S.
dc.contributor.authorVillagra, N.
dc.contributor.authorFuentes, J.
dc.date.accessioned2021-07-12T14:29:10Z
dc.date.available2021-07-12T14:29:10Z
dc.date.issued2006-05
dc.descriptionIndexación: Scopus.es
dc.description.abstractThe Salmonella enterica tsx gene encodes a nucleoside-specific outer membrane channel. The Tsx porin is essential for the prototrophic growth of S. enterica sv. Typhi in the absence of nucleosides. RT-PCR analysis shows that the tsx gene is cotranscribed with an open reading frame unique to S. enterica, impX (STY0450), which encodes an inner membrane protein 108 amino acids in length, which is predicted to have only two transmembrane α-helices. Fusions of the lacZ gene to both tsx and impX reveal that the transcription of both genes is induced in the presence of adenosine. A null mutation in the S. Typhi impX gene suppresses the induced auxotrophy for adenosine or thymidine resulting from a tsx mutation and confers sensitivity to high concentrations of adenosine or thymidine. The ImpX protein, when tagged with a 3xFLAG epitope, is functional and associates with the inner membrane; impX mutants are defective in the export of 3H-radiolabeled thymidine. Taken together, these and other results suggest that the S. Typhi Tsx porin and ImpX inner membrane protein facilitate competing mechanisms of thymidine influx and efflux, respectively, to maintain the steady-state levels of internal nucleoside pools.es
dc.description.urihttps://academic.oup.com/genetics/article/173/1/25/6061595
dc.identifier.citationGenetics, Volume 173, Issue 1, Pages 25 - 34, May 2006es
dc.identifier.doiDOI: 10.1534/genetics.105.054700
dc.identifier.issn0016-6731
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/19346
dc.language.isoenes
dc.publisherGenetics Society of America [Society Publisher] Oxford University Press [University Publisher]es
dc.subjectAdenosinees
dc.subjectBacterial Outer Membrane Proteinses
dc.subjectDNAes
dc.subjectBacteriales
dc.subjectEpistasises
dc.subjectProtein Transportes
dc.subjectSalmonella entericaes
dc.subjectSalmonella typhies
dc.subjectSuppressiones
dc.subjectGenetices
dc.titleThe cotranscribed Salmonella enterica sv. Typhi Tsx and impX genes encode opposing nucleoside-specific import and export proteinses
dc.typeArtículoes
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