Co-Culture of P. gingivalis and F. nucleatum Synergistically Elevates IL-6 Expression via TLR4 Signaling in Oral Keratinocytes

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dc.contributor.authorYáñez, Lucas
dc.contributor.authorSoto, Cristopher
dc.contributor.authorTapia, Héctor
dc.contributor.authorPacheco, Martín
dc.contributor.authorTapia, Javiera
dc.contributor.authorOsses, Gabriela
dc.contributor.authorSalinas, Daniela
dc.contributor.authorRojas-Celis, Victoria
dc.contributor.authorHoare, Anilei
dc.contributor.authorQuest, Andrew F. G.
dc.contributor.authorDíaz-Elizondo, Jessica
dc.contributor.authorPérez-Donoso, José Manuel
dc.date.accessioned2024-05-09T14:39:31Z
dc.date.available2024-05-09T14:39:31Z
dc.date.issued2024-04-07
dc.descriptionIndexación: Scopus.
dc.description.abstractPeriodontitis, characterized by persistent inflammation in the periodontium, is intricately connected to systemic diseases, including oral cancer. Bacteria, such as Porphyromonas gingivalis and Fusobacterium nucleatum, play a pivotal role in periodontitis development because they contribute to dysbiosis and tissue destruction. Thus, comprehending the interplay between these bacteria and their impacts on inflammation holds significant relevance in clinical understanding and treatment advancement. In the present work, we explored, for the first time, their impacts on the expressions of pro-inflammatory mediators after infecting oral keratinocytes (OKs) with a co-culture of pre-incubated P. gingivalis and F. nucleatum. Our results show that the co-culture increases IL-1β, IL-8, and TNF-α expressions, synergistically augments IL-6, and translocates NF-kB to the cell nucleus. These changes in pro-inflammatory mediators—associated with chronic inflammation and cancer—correlate with an increase in cell migration following infection with the co-cultured bacteria or P. gingivalis alone. This effect depends on TLR4 because TLR4 knockdown notably impacts IL-6 expression and cell migration. Our study unveils, for the first time, crucial insights into the outcomes of their co-culture on virulence, unraveling the role of bacterial interactions in polymicrobial diseases and potential links to oral cancer.
dc.description.urihttps://www-scopus-com.recursosbiblioteca.unab.cl/record/display.uri?eid=2-s2.0-85190442411&origin=resultslist&sort=plf-f&src=s&nlo=&nlr=&nls=&sid=ccb1b0f08bac451e740b63ee5790da6a&sot=aff&sdt=cl&cluster=scofreetoread%2c%22all%22%2ct&sl=61&s=AF-ID%28%22Universidad+Andr%c3%a9s+Bello%22+60002636%29+AND+SUBJAREA%28COMP%29&relpos=5&citeCnt=0&searchTerm=
dc.identifier.citationInternational Journal of Molecular Sciences Open Access Volume 25, Issue 7 April 2024 Article number 3611
dc.identifier.doi10.3390/ijms25073611
dc.identifier.issn16616596
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/56657
dc.language.isoen
dc.publisherMDPI
dc.rights.licenseCC BY 4.0 DEED Attribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectco-culture
dc.subjectcytokines
dc.subjectFusobacterium nucleatum
dc.subjectinflammation
dc.subjectoral cancer
dc.subjectperiodontitis
dc.subjectPorphyromonas gingivalis
dc.subjecttoll-like receptor 4
dc.titleCo-Culture of P. gingivalis and F. nucleatum Synergistically Elevates IL-6 Expression via TLR4 Signaling in Oral Keratinocytes
dc.typeArtículo
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