Forward genetic screens in zebrafish identify pre-mRNA-processing pathways regulating early T cell development
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Fecha
2016-11
Profesor/a Guía
Facultad/escuela
Idioma
en
Título de la revista
ISSN de la revista
Título del volumen
Editor
Elsevier B.V.
Nombre de Curso
Licencia CC
Atribution 4.0 International (CC BY 4.0)
Licencia CC
https://creativecommons.org/licenses/by/4.0/deed.es
Resumen
ymphocytes represent basic components of verte brate adaptive immune systems, suggesting the util ity of non-mammalian models to define the molecular
basis of their development and differentiation. Our
forward genetic screens in zebrafish for recessive
mutations affecting early T cell development re vealed several major genetic pathways. The iden tification of lineage-specific transcription factors
and specific components of cytokine signaling and
DNA replication and/or repair pathways known
from studies of immunocompromised mammals pro vided an evolutionary cross-validation of the screen
design. Unexpectedly, however, genes encoding
proteins required for pre-mRNA processing were en riched in the collection of mutants identified here. In
both zebrafish and mice, deficiency of the splice
regulator TNPO3 impairs intrathymic T cell differenti ation, illustrating the evolutionarily conserved and
cell-type-specific functions of certain pre-mRNA processing factors for T cell development.
Notas
Indexación: Scopus.
Palabras clave
T Cell Development, Genetic Screen, Immunodeficiency, Zebrafish, Mouse, Pre-mRNA, Splicing
Citación
Cell Reports. Volume 17, Issue 9, Pages 2259 - 2270. 22 November 2016
DOI
DOI: 10.1016/j.celrep.2016.11.003