Estudio in vitro, citotoxicidad de cementos de resina auto-adhesivos y auto-grabantes sobre células madre
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Fecha
2013
Autores
Profesor/a Guía
Facultad/escuela
Idioma
es
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Editor
Universidad Andrés Bello
Nombre de Curso
Licencia CC
Licencia CC
Resumen
El objetivo del estudio es investigar la toxicidad de los cementos de resina autoadhesivos
y auto-grabantes en cultivos de células madres de la pulpa dental.
Los cementos utilizados fueron el seT (SOl) y el RelyX U200 (3M ESPE). Se realizó una
elución de estos cementos por un período de 24 horas a 37°C, posteriormente se
usaron concentraciones de 100%,75%, 50% y 25%.
El test de citotoxicidad se realizó en cultivos de células madres, usando las 4
concentraciones de cada elución, realizando la incubación a 37°C, en ambiente húmedo
y 5% de CO2 por 48 horas.
La citotoxicidad se analizó morfológicamente usando el método ISO 10993 Parte 5, y
cuantitativamente usando citometría de flujo.
Resultados: A nivel de microscopía óptica ambos cementos resultaron ser citotóxicos
para las células madres observándose cambios morfológicos drásticos y alto índice de
muerte celular, a excepción de la concentración al 25% que no tuvo una diferencia
estadísticamente significativa con el control.
En el análisis cuantitativo, todas las concentraciones de seT y RelyX U200 fueron
tóxicas para las células madres, afectando el índice de viabilidad, el cual disminuyó
significativamente en todos los tratamientos; aunque el carácter genotípico de estas
células no se perdió.
Estos resultados sugieren aplicar con discreción el cemento en cavidades profundas, ya
que si un eluido llegase a la cavidad pulpar causaría toxicidad severa sobre las células
madres pulpares disminuyendo la probabilidad de reparación mediante la aposición de
dentina terciaria frente a una noxa de la pieza dental.
The objective of this study was to investigate the toxicity of the self-adhesives and self etching resin cements. The cement used were seT (SOl) and RelyXU200 (3M ESPE). These cement were eluted for 24 hours at 37°C ( +/- 1 °C) and 100%, 75%, 50% and 25% dilutions were used. Cytotoxicity test was done on stem cell cultures, treated with each dilution of the cement eluted, and incubated at 37°C, with humidity and 5% C02 for 48 hours. At the morphological level, the cytotoxicity was evaluated by the ISO 10993 Part 5, while flow cytometry was used for quantitative analyses. Results: At the morphological level cytotoxicity was observed in all the treatments except at 25% with both cements compared to the controls. High mortality and large morphological change were observed. The quantitative analyses show cytotoxicity with both cements seT and RelyX U200 in all the concentrations used. The cell viability was affected with all the treatments, while the stem cell character remained without change. These results suggest a discretional use of the cements that come into close and prolonged contact with pulpo-dentin complex, to prevent the cytotoxicity on human dental pulp stem cells. The pulp stem cell damage could lead to poor performance of the pulp dentin complex against injuries due to the lack of reparative dentin.
The objective of this study was to investigate the toxicity of the self-adhesives and self etching resin cements. The cement used were seT (SOl) and RelyXU200 (3M ESPE). These cement were eluted for 24 hours at 37°C ( +/- 1 °C) and 100%, 75%, 50% and 25% dilutions were used. Cytotoxicity test was done on stem cell cultures, treated with each dilution of the cement eluted, and incubated at 37°C, with humidity and 5% C02 for 48 hours. At the morphological level, the cytotoxicity was evaluated by the ISO 10993 Part 5, while flow cytometry was used for quantitative analyses. Results: At the morphological level cytotoxicity was observed in all the treatments except at 25% with both cements compared to the controls. High mortality and large morphological change were observed. The quantitative analyses show cytotoxicity with both cements seT and RelyX U200 in all the concentrations used. The cell viability was affected with all the treatments, while the stem cell character remained without change. These results suggest a discretional use of the cements that come into close and prolonged contact with pulpo-dentin complex, to prevent the cytotoxicity on human dental pulp stem cells. The pulp stem cell damage could lead to poor performance of the pulp dentin complex against injuries due to the lack of reparative dentin.
Notas
Tesis (Cirujano Dentista, Especialización en Biomateriales Dentales)
Palabras clave
Materiales Dentales, Adhesivos Dentales, Células Madre, Chile