Molecular determinants for cyclo-oligosaccharide-based nanoparticle-mediated effective siRNA transfection

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dc.contributor.authorManzanares, Darío
dc.contributor.authorAraya-Durán, Ingrid
dc.contributor.authorGallego-Yerga, Laura
dc.contributor.authorJátiva, Pablo
dc.contributor.authorMárquez-Miranda, Valeria
dc.contributor.authorCanan, Jonathan
dc.contributor.authorJiménez Blanco, José Luis
dc.contributor.authorMellet, Carmen Ortiz
dc.contributor.authorGonzález-Nilo, Fernando Danilo
dc.contributor.authorGarcía Fernández, José Manuel
dc.contributor.authorCeña, Valentín
dc.date.accessioned2024-04-30T15:33:09Z
dc.date.available2024-04-30T15:33:09Z
dc.date.issued2017-07
dc.descriptionIndexación: Scopus
dc.description.abstractAim: To study the structural requirements that a cyclooligosaccharide-based nanoparticle must fulfill to be an efficient siRNA transfection vector. Materials & methods: siRNA protection from degradation by RNAses, transfection efficiency and the thermodynamic parameters of the nanoparticle/siRNA interactions were studied on pairs of amphiphilic molecules using biochemical techniques and molecular dynamics. Results: The lower the siRNA solvent accessible surface area in the presence of the nanoparticle, higher the protection from RNAse-mediated degradation in the corresponding nanocomplex; a moderate nanoparticle/siRNA binding energy value further facilitates reversible complexation and binding to the target cellular mRNA. Conclusion: The use, in advance, of these parameters will provide a useful indication of the potential of a molecular nanoparticle as siRNA transfecting vector. © 2017 Future Medicine Ltd.
dc.description.urihttps://www.futuremedicine.com/doi/10.2217/nnm-2017-0123
dc.identifier.citationNanomedicine Volume 12, Issue 13, Pages 1607 - 1621July 2017
dc.identifier.doi10.2217/nnm-2017-0123
dc.identifier.issn1743-5889
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/56487
dc.language.isoen
dc.publisherFuture Medicine Ltd.
dc.rights.licenseCC BY-NC-ND 4.0 DEED Atribución-NoComercial-SinDerivadas 4.0 Internacional
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subjectendosomal escape
dc.subjectmolecular modeling
dc.subjectmolecular nanoparticle
dc.subjectprotection from RNAses
dc.subjectprotein knock down
dc.subjectsiRNA transfection
dc.titleMolecular determinants for cyclo-oligosaccharide-based nanoparticle-mediated effective siRNA transfection
dc.typeArtículo
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