Gold nanorods/siRNA complex administration for knockdown of PARP-1: A potential treatment for perinatal asphyxia

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dc.contributor.authorVio, Valentina
dc.contributor.authorRiveros, Ana L.
dc.contributor.authorTapia-Bustos, Andrea
dc.contributor.authorLespay-Rebolledo, Carolyne
dc.contributor.authorPérez-Lobos, Ronald
dc.contributor.authorMuñoz, Luis
dc.contributor.authorPismante, Paola
dc.contributor.authorMorales, Paola
dc.contributor.authorAraya, Eyleen
dc.contributor.authorHassan, Natalia
dc.contributor.authorHerrera-Marschitz, Mario
dc.contributor.authorKogan, Marcelo J.
dc.date.accessioned2022-09-09T13:03:34Z
dc.date.available2022-09-09T13:03:34Z
dc.date.issued2018
dc.descriptionIndexación Scopuses
dc.description.abstractBackground: Perinatal asphyxia interferes with neonatal development, resulting in long-term deficits associated with systemic and neurological diseases. Despite the important role of poly (ADP-ribose) polymerase 1 (PARP-1) in the regulation of gene expression and DNA repair, overactivation of PARP-1 in asphyxia-exposed animals worsens the ATP-dependent energetic crisis. Inhibition of PARP-1 offers a therapeutic strategy for diminishing the effects of perinatal asphyxia. Methods: We designed a nanosystem that incorporates a specific siRNA for PARP-1 knockdown. The siRNA was complexed with gold nanorods (AuNR) conjugated to the peptide CLPFFD for brain targeting. Results: The siRNA was efficiently delivered into PC12 cells, resulting in gene silencing. The complex was administered intraperitoneally in vivo to asphyxia-exposed rat pups, and the ability of the AuNR-CLPFFD/siRNA complex to reach the brain was demonstrated. Conclusion: The combination of a nanosystem for delivery and a specific siRNA for gene silencing resulted in effective inhibition of PARP-1 in vivo. © 2018 Vio et al.es
dc.description.urihttps://www.dovepress.com/gold-nanorodssirna-complex-administration-for-knockdown-of-parp-1-a-po-peer-reviewed-fulltext-article-IJN#
dc.identifier.citationInternational Journal of Nanomedicine Volume 13, Pages 6839 - 6854 2018es
dc.identifier.doi10.2147/IJN.S175076
dc.identifier.issn11769114
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/23820
dc.language.isoenes
dc.publisherDove Medical Press Ltd.es
dc.rights.licenseCC BY-NC 4.0
dc.subjectADP-ribosylationes
dc.subjectPoly ADP-ribose Glycohydrolasees
dc.subjectDNA Damagees
dc.subjectGold nanorodses
dc.subjectIn vivo administrationes
dc.subjectNeonatal hypoxiaes
dc.subjectsiRNA deliveryes
dc.titleGold nanorods/siRNA complex administration for knockdown of PARP-1: A potential treatment for perinatal asphyxiaes
dc.typeArtículoes
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